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有报告帕金森氏病患者的脑中5羟色胺含量减少50～60%,但是尚无合成酶-色氨酸羟化酶改变的报道。作者应用有荧光检测器的高压液相色谱仪测定了帕金森氏病、纹状体黑质变性、Shy-Drager综合征和进行性核上性麻痹患者与对照组患者脑中的色氨酸羟化酶活性。色氨酸羟化酶催化L-色氨酸生成L-5羟色胺,这是5羟色胺神经元和松果体中黑色素生物合成的起始和限速步骤。与其它动物种属相比,人脑中该酶的活性很低且不稳定。作者取患者死后2～15小时内的脑标本,然后储存在-80℃,对照组患者无神经系统疾病史,亦未用过任何影响酶活性的药物。将脑组织匀浆液作为酶源,测定色氨酸羟化酶活性。结果发现对照组脑中酶活性的分布部位与5羟色胺神经元的分布部位相同。
It has been reported that the Parkinson’s disease in patients with brain serotonin reduced by 50 ~ 60%, but no change of synthetase - tryptophan hydroxylase reported. The authors used high-pressure liquid chromatography with a fluorescence detector to measure the levels of tryptophan in the brains of patients with Parkinson’s disease, striatonigral degeneration, Shy-Drager syndrome and patients with progressive supranuclear palsy Enzymatic activity. Tryptophan hydroxylase catalyzes the formation of L-serotonin by L-tryptophan, which is the initial and rate-limiting step in melanin biosynthesis in serotonin neurons and pineal glands. Compared to other animal species, the activity of this enzyme in the human brain is low and unstable. The authors took brain samples from 2 to 15 hours after their death and then stored at -80 ° C. The control group had no history of neurological diseases and no drugs that affected enzyme activity. Brain tissue homogenate as enzyme source, tryptophan hydroxylase activity. The results showed that the distribution of enzymatic activity in the brain of the control group was the same as that of serotonin neurons.