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【目的】筛选适合用于构建割手密遗传连锁图谱的分离群体,为后续的遗传连锁图谱构建和基因定位及遗传育种提供理论依据。【方法】基于“双假测交”理论,利用DNA水平上遗传差异较大且蔗糖分和分蘖率差异极显著的不同割手密无性系进行杂交获得3个不同杂交组合,对3个备选作图群体的分蘖率和蔗糖分进行变异、正态分布拟合分析及分子多态性分析。【结果】组合3(GXS85-12×GXS87-16)和组合13(云割①×田林)的分蘖率呈偏态分布,而组合5(GXS85-30×GXS87-16)的分蘖率呈近似正态分布;组合3和组合5的蔗糖分呈偏态分布,而组合13的蔗糖分呈近似正态分布。在分子多态性水平上,组合5亲本的遗传相似系数略高于组合3和组合13,而其后代平均遗传相似系数略低,但变幅较高。【结论】组合5(GXS85-30×GXS87-16,群体大小为245株)的分蘖率、蔗糖分遗传性相对稳定,群体来源比较广泛,分子多态性丰富,遗传多样性高,可作为分离群体用于构建遗传图谱。
【Objective】 The objective of this study was to screen for the segregation population suitable for constructing the genetic map of Qiemi map and to provide the theoretical basis for the construction of genetic linkage map, genetic mapping and genetic breeding. 【Method】 Based on the theory of “double fake test”, three different crosses were obtained by using different clonal clones with large genetic differences in DNA level and extremely different sucrose content and tillering rate, The tillering rate and sucrose content of the alternate mapping population were analyzed by fitting, normal distribution fitting analysis and molecular polymorphism. 【Result】 The results showed that the tillering rates of combination 3 (GXS85-12 × GXS87-16) and combination 13 (mulching ① × Tianlin) showed a skewed distribution, whereas that of combination 5 (GXS85-30 × GXS87-16) The distribution of sucrose content in combination 3 and combination 5 was skewed, while that of combination 13 was approximately normal distribution. At the level of molecular polymorphism, the genetic similarity coefficient of combination 5 parents was slightly higher than that of combination 3 and combination 13, while the average genetic similarity coefficient of their offspring was slightly lower but the amplitude was higher. 【Conclusion】 The tillering rate and sucrose heredity of combination 5 (GXS85-30 × GXS87-16, population size 245) were relatively stable, the sources of their populations were relatively extensive, their molecular polymorphisms were abundant and their genetic diversity was high, The population is used to construct the genetic map.