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In the present study,a Si-containing coating was fabricated on AZ31 B Mg alloy.Cytocompatibility of the coated alloy was evaluated by both indirect and direct contact methods,respectively.Effects of a number of incubation variables on the sensitivity and reproducibility of the hemolysis test were also examined by using positively and negatively responding biomaterials.Cytocompatibility testing results indicated that cell condition,cell adherence,cell proliferation and extracellular matrix secretion of the coated alloy were improved compared with those of the uncoated alloy for different extraction and co-culture time.The hemolysis test suggested that hemolysis testing conditions were critical to determine the hemolysis of the alloy.It was also found that 1 day in vitro degradation of the uncoated AZ31 B alloy had no destructive effect on erythrocyte.As for the coated AZ31 B alloy at any time point,the hemolysis rate was much lower than 5%,the safe value for biomaterials.These in vitro experimental results indicate that the Si-containing coating is effective to improve the cytocompatibility and hemolysis behaviors of AZ31 B alloy during its degradation.
In the present study, a Si-containing coating was fabricated on AZ31 B Mg alloy. Cytocompatibility of the coated alloy was evaluated by both indirect and direct contact methods, respectively. Effects of a number of incubation variables on the sensitivity and reproducibility of the hemolysis test were also examined by using positively and negatively responding biomaterials. Cytocompatibility testing results indicated that cell condition, cell adherence, cell proliferation and extracellular matrix secretion of the coated alloy were improved compared with those of the uncoated alloy for different extraction and co-culture time The hemolysis test suggested that hemolysis testing conditions were critical to determine the hemolysis of the alloy. It was also found that 1 day in vitro degradation of the uncoated AZ31 B alloy had no destructive effect on erythrocyte. As for the coated AZ31 B alloy at any time point, the hemolysis rate was much lower than 5%, the safe value for biomaterials. These in vitro experimental results indicate that the Si-containing coating is effective to improve the cytocompatibility and hemolysis behaviors of AZ31 B alloy during its degradation.