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以高抗CMV的烤烟品种台烟8号为母本(P1),以高感CMV的烤烟品种NC82为父本(P2),在2个不同的时间环境下构建P1、P2、F1和F24个世代群体,在植株不同生长时期进行CMV病害鉴定。运用植物数量性状“主基因+多基因”混合遗传模型分析方法对该世代群体的CMV抗性进行联合分析。结果表明,在温室环境中,苗期和成株期鉴定CMV抗性遗传都符合E1模型,即由2对加性-显性-上位性主基因+加性-显性多基因混合控制,主基因遗传率分别是37.11%、57.76%;在大田环境中,苗期抗性鉴定符合加性-显性-上位性多基因模型(C0),多基因遗传率为26.86%,而成株期鉴定属于2对主基因+多基因模型(E2),主基因遗传率为36.57%。研究表明,由于植物抗性基因的表达具有时空性,台烟8号对CMV的抗性遗传在不同的时间和环境具有一定的差异;但随着植株的生长,抗性遗传趋于稳定,在成株期时,2个不同的环境均表现为2对主基因+多基因控制,所以对烤烟CMV抗性品种选育和改良要以主基因为主,同时注重环境的影响。
(P1) with high anti-CMV flue-cured tobacco variety Typhoon No. 8 and parents (P2) with high susceptibility to flue-cured tobacco variety NC82 as CMV, and constructed P1, P2, F1 and F24 in two different time environments Generation groups, CMV disease identification in different growth stages of plants. The CMV resistance of this offspring population was analyzed using the quantitative trait of plants and the analysis of “major gene + polygene” mixed genetic model. The results showed that in the greenhouse environment, the inheritance of CMV resistance in seedling stage and adult stage accorded with the E1 model, that is, it was controlled by two pairs of additive-dominance-epistatic major genes plus additive-dominant polygenes, The gene heritability was 37.11% and 57.76%, respectively. In field environment, the seedling stage resistance accorded with additive-dominance-epistatic polygene model (C0), and the polygene heritability was 26.86% Belong to 2 pairs of major genes + polygene model (E2), the main gene heritability was 36.57%. The results showed that the genetic variation of CMV in Taiyan 8 was different at different times and environments because of the spatiotemporal expression of plant resistance genes. However, with the growth of plants, the resistance inheritance tended to be stable. At the adult stage, two major environments were controlled by two major genes plus polygenes. Therefore, the selection and improvement of CMV resistant flue-cured tobacco should be dominated by major genes while paying attention to the environmental impact.