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用小剂量多次腹腔注射链佐霉素的方法破坏胰岛β细胞,造成小鼠糖尿病,其主要表现为血糖升高和血清胰岛素浓度下降。在每次注射链佐霉素前10—15min,由皮下注射 CCK-8(100μg/kg),可使链佐霉素的损伤作用減轻或不出现:小鼠血糖浓度由对照组的518.9±53.6mg%降低到267.1±16.8mg%,糖尿病发病率由83.3%降到30%;血清胰岛素浓度基本正常,表明 CCK-8对链佐霉素引起的糖尿病有一定的预防作用。用链佐霉素造成高血糖后,再用 CCK-8作治疗性注射,不能减轻高血糖的程度,表明 CCK-8无治疗作用。CCK-8对正常小鼠的血糖及胰岛素水平也无明显影响。以上结果提示,CCK-8对胰岛β细胞可能具有直接保护作用。
Small doses of multiple intraperitoneal injection of streptozotocin method of destruction of islet β cells, resulting in diabetes in mice, which is mainly manifested as elevated blood glucose and serum insulin levels decreased. The subcutaneous injection of CCK-8 (100μg / kg) 10-30min before each injection of streptozotocin can reduce or not show the damaging effect of streptozotocin: the blood glucose concentration in mice was increased from 518.9 ± 53.6mg% was reduced to 267.1 ± 16.8mg%, the incidence of diabetes was reduced from 83.3% to 30%, serum insulin concentration was basically normal, which indicated that CCK-8 could prevent streptozotocin-induced diabetes. CCK-8 as a therapeutic injection after hyperglycemia caused by streptozotocin, can not reduce the degree of hyperglycemia, indicating that CCK-8 has no therapeutic effect. CCK-8 on normal mice, blood glucose and insulin levels also had no significant effect. The above results suggest that CCK-8 may have a direct protective effect on pancreatic β-cells.