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目的研究CCR5 59029等位基因在贵州世居少数民族(彝、瑶族)以及汉族人群的分布,并分析CCR5位点突变与乙型肝炎病毒(HBV)的关系。方法 2009年2月至5月,采用PCR-RFLP技术分析贵州92份黔西彝族、101份威宁彝族、138份荔波瑶族以及165份毕节汉族CCR5启动子区59029位点多态性,抽取样本进行DNA测序验证。结果贵州省黔西彝族、威宁彝族、荔波瑶族及毕节汉族的CCR559029位点基因型频率分别为:纯合突变型(AA)为20.65%、17.82%、8.7%、22.42%;杂合子(AG)为43.48%、44.55%、34.78%、44.85%;野生型(GG)为35.87%、37.62%、56.52%、32.37%。等位基因频率:A为26.09%～44.85%,G为55.15%～73.91%。基因型频率在荔波瑶族和民族总体人群感染组与非感染组之间分布有统计学意义(P<0.05),等位基因频率在威宁彝族、荔波瑶族人群感染组与非感染组之间分布差异有统计学意义(P<0.05)。结论 CCR5 59029位点可能对贵州省汉、瑶、彝族人群HBV感染具有保护性,CCR5基因可能是HBV感染的拮抗基因之一。
Objective To investigate the distribution of CCR5 59029 allele in Guizhou ethnic minorities (Yi, Yao) and Han population, and to analyze the relationship between CCR5 locus mutation and hepatitis B virus (HBV). Methods From February to May 2009, PCR-RFLP was used to analyze the polymorphism of 59029 locus in 92 parts of Guizhou province, 101 parts of Weining Yi, 138 parts of Libo Yao and 165 parts of Bijie Han. Sample DNA sequencing validation. Results The genotype frequency of CCR559029 in Qianxi Yi, Weining Yi, Libo Yao and Bijie Han in Guizhou Province were 20.65%, 17.82%, 8.7% and 22.42%, respectively. The heterozygotes AG) were 43.48%, 44.55%, 34.78% and 44.85%, respectively. The wild type (GG) was 35.87%, 37.62%, 56.52% and 32.37% respectively. Allele frequency: A was 26.09% ~ 44.85%, G was 55.15% ~ 73.91%. The frequencies of genotypes were statistically significant between infected and non-infected groups in Libo and ethnic groups (P <0.05). The frequencies of alleles were significantly higher in non-infected and non-infected groups The differences between the two groups were statistically significant (P <0.05). Conclusion CCR5 59029 may be protective against HBV infection among Han, Yao and Yi ethnic groups in Guizhou Province. CCR5 may be one of the antagonists of HBV infection.