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由新的半矮秆品系RA73与其它4个不同株高的品种Lemont（半矮秆）、L202（次半矮秆）、Katy（次半矮秆）和Bond（高秆）组成一组完全双列杂交试验。于1994年和1995年对亲本、F1和F2进行中子辐射而得到的半矮秆品系RA73，控制其株高的基因，与半矮秆品种Lemont具有的sd—1半矮秆基因是不等位的。RA73与Lemont、L202和Katy杂交，其F2代的分离比为：9高秆：6半矮秆：1特矮秆或9高秆：3次半矮秆：3半矮秆：1特矮秆。在这些组合中存在两对不等位的独立的半矮秆基因。F1和F2出现高秆野生型植株，F2还出现新的双隐基因控制的特矮秆植株。L202或Katy具有显性次半矮秆基因，Lemont具隐性半矮秆基因。L202与Katy杂交，F2的株高为正态的数量性状连续分布，都具有相同的次半矮秆基因。高秆品种Bond与其它4个次半矮秆或半矮秆品种杂交，F2株高的分离比为：3高秆：1次半矮秆或半矮秆，表明由显隐性等位基因控制遗传。正交和反交的结果一致，存在的差异，主要是环境的影响，除主基因外，还存在微效多基因的修饰基因，但不是母性效应。
The new semi-dwarf strain RA73 is composed of a complete set of four pairs of Lemont (semi-dwarf), L202 (semi-dwarf), Katy (semidwarf) and Bond Column hybridization test. The semi-dwarf strain RA73 obtained from neutron irradiation on parents, F1 and F2 in 1994 and 1995 was different from the sd-1 semi-dwarf gene in semi-dwarf cultivars Lemont Bit. RA73 was crossed with Lemont, L202 and Katy. The segregation ratio of F2 generation was: 9 Tall: 6 Semi-dwarf: 1 dwarf or 9 tall: 3 dwarf: 3 dwarf: 1 dwarf . In these combinations there are two pairs of independent dwarfing genes that are not in the same position. F1 and F2 appeared high-stalk wild-type plants, F2 also appeared a new dwarf gene-controlled dwarf plants. L202 or Katy has a dominant semi-dwarf gene and Lemont has a recessive dwarf gene. L202 was crossed with Katy. The plant height of F2 was continuous with the normal quantitative trait and all had the same sub-dwarf gene. The high-stalk variety Bond was crossed with four other semi-dwarf or semi-dwarf cultivars. The segregation ratio of F2 plants was 3 tall stalk: 1 semi-dwarf or semi-dwarf stalk, indicating that it was controlled by the dominant recessive allele Genetic. Orthogonal and anti-cross the same results, differences exist, mainly the impact of the environment, in addition to the main genes, there are micro-gene modified genes, but not the maternal effect.