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目的观察田鼠巴贝虫(Babesia microti)隐性感染期小鼠经再感染、免疫抑制或盲传健康小鼠后的外周血红细胞内虫体密度的消长规律。方法取1只感染种鼠的外周血(虫密度为20%),腹腔接种6周龄雌性BALB/c健康小鼠12只,100μl/只,用随机数字表法分为对照组、再感染组、免疫抑制组和盲传组,每组3只。4组感染小鼠连续28 d尾部采血,吉氏染色法观察田鼠巴贝虫形态变化,并计算红细胞染虫率,构建隐性感染小鼠模型。再感染组隐性感染小鼠再次腹腔接种相同剂量的田鼠巴贝虫感染种鼠血;免疫抑制组隐性感染小鼠腹腔注射地塞米松,0.5 mg/只,连续注射5 d;盲传组3只隐性感染小鼠取眼眶血,分别腹腔盲传接种健康雌性BALB/c小鼠各3只,100μl/只。3组小鼠继续尾部采血28 d,镜下计数感染红细胞,计算染虫率,并观察田鼠巴贝虫形态变化。结果对照组、再感染组、免疫抑制组和盲传组小鼠外周血均于感染后第3天查见田鼠巴贝虫体,第7天红细胞染虫率最高,分别为73.2%、78.0%、76.2%及79.0%,随后染虫率逐渐下降,至第28天外周血镜检阴性,进入隐性感染阶段。再感染组小鼠再次感染后28 d内,每天染虫率均为0。免疫抑制组小鼠在免疫抑制后第2天查见虫体,第12天染虫率再次达到高峰,为65.2%,随后逐渐下降,至第22天再次进入隐性感染期。盲传组新感染的9只小鼠在感染后第4天查见虫体,第9~10天染虫率达到高峰,为35.0%~39.0%,随后逐渐下降,至第28天小鼠进入隐性感染阶段。各组感染的田鼠巴贝虫形态变化基本一致,染虫初期以小环状体居多;染虫高峰期多见大环状体和长丝状体;有多虫寄生现象。结论田鼠巴贝虫隐性感染期小鼠有带虫免疫现象,并可作为传染源;经免疫抑制后虫体被激化,可出现与首次感染相同的虫体密度消长规律。
Objective To observe the growth and decline of peripheral blood erythrocyte density in re-infected, immunosuppressed or blindly passaged mice of Babesia microti during recessive infection. Methods Peripheral blood (20%) of infected mice was inoculated intraperitoneally. Twelve female BALB / c healthy mice (6 weeks old) were intraperitoneally inoculated into the control group with random number table. The re-infected group , Immunosuppressive group and blind transmission group, 3 in each group. Four groups of infected mice were continuously fed blood for 28 days, and the morphological changes of Babesia microphyllae were observed by Giemsa staining. The rate of red blood cells was calculated, and the latent infection mouse model was constructed. Inoculated mice were inoculated intraperitoneally with the same dose of Babesia vivax to infect the mice blood. The mice in the immunosuppressive group were inoculated intraperitoneally with dexamethasone 0.5 mg / Three orbital infected mice were blindly passaged, and three female BALB / c mice were immunized intraperitoneally blindly (100μl / body). Three groups of mice continue to tail blood sampling 28 d, count erythrocytes counting microscopy, counting the rate of infection, and observe the morphological changes of voles Babies. Results In the control group, re-infected group, immunosuppressive group and blind transfer group mice peripheral blood were seen on the 3rd day after infection, the Labiatae voles was found, the highest rate of red blood cells on the 7th day were 73.2%, 78.0% , 76.2% and 79.0%, respectively. Then the rate of coccidiosis decreased gradually, and the peripheral blood microscopy was negative on the 28th day and entered the latent infection stage. Within 28 days after re-infection, the rate of infection by penicillin was 0 every day. The immunosuppressive mice were found on the second day after immunosuppression. The coccidia reached a peak of 65.2% again on the 12th day, then decreased gradually and reached latent infection again on the 22nd day. The newly infected 9 mice in Blind group were found on the 4th day after infection, and the highest incidence rate was 35.0% -39.0% on the 9th to 10th day, and then decreased gradually. By the 28th day, the mice entered Recessive infection stage. The morphological changes of Babesia in voles infected by each group were basically the same, the majority of them were small-ring-shaped ones in the early stage of the infection; the macrocycles and filamentous bodies were more common in the peak of the infection; CONCLUSION: The mice immunized with Babesia vaporiza during the latent infection period showed the phenomenon of parasitic immunity and could be used as the source of infection. After immunosuppression, the parasites were exacerbated and the same pattern of growth and decline of the parasites could occur.