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使用环介导恒温扩增技术,基于莱姆病病原伯氏疏螺旋体的外膜蛋白A(OspA)基因,针对伯氏疏螺旋体不同的基因型设计特异性引物,对国内主要的莱姆病病原伯氏疏螺旋体的3个基因型进行分型鉴定。研究结果表明,设计的引物具有良好的特异性,可以对狭义伯氏疏螺旋体(Borrelia burgdorferi sensu strict)、嘎氏疏螺旋体(B.afzelii)和伽氏疏螺旋体(B.garinii)进行分型鉴定。伯氏疏螺旋体的分型鉴定可以对不同临床症状莱姆病患者的治疗和莱姆病的控制提供一定的依据。
Using ring-mediated isothermal amplification technique, specific primers were designed for different genotypes of Borrelia burgdorferi based on the outer membrane protein A (OspA) gene of Lyme disease Borrelia burgdorferi, The three genotypes of Borrelia burgdorferi were identified by typing. The results showed that the designed primers had good specificity and could be used to identify the narrow Borrelia burgdorferi sensu strict, B. afzelii and B. garinii . The identification of Borrelia burgdorferi can provide some evidences for the treatment of Lyme disease and the control of Lyme disease in different clinical symptoms.