A new procedure for developing high performance enzyme electrode is described. Theprocedure is based on the covalently binding of horseradish Peroxidase to a self assemblingmonolayer on a gold electrode. An enhanced catalytic current is observed with addihon of hydro-gen peroxide into phosphate buffer solution (PBS) in the presence of mediators. The electrodeexhibits high sensihvity and a wide linear range to hydrogen peroxide. A new procedure for developing high performance enzyme electrode is described. The covalently binding of horseradish peroxidase to a self assembling monolayer on a gold electrode. An enhanced catalytic current is observed with addihon of hydro-gen peroxide into phosphate buffer solution ) in the presence of mediators. The electrode exhibits high sensihvity and a wide linear range to hydrogen peroxide.