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目的:基于均匀设计方法研究多种中药有效组分体外清除致癌物多环芳烃的最佳配伍作用。方法:采用改进最小角(least angle regression,LARS)回归分析,通过荧光光谱法进行甘草总黄酮、银杏叶提取物、淫羊藿总黄酮和黄芪总苷4个中药组分不同配比清除多环芳烃的作用研究。结果:通过LRAS回归分析获得了中药组分配伍清除多环芳烃的回归方程,优化组合后进行药效学验证,结果显示该中药组分配伍最佳剂量组合为甘草总黄酮3.144 g·L-1、银杏叶提取物0.8 g·L-1、淫羊藿总黄酮0.024 g·L-1和黄芪总苷0.036 g·L-1。结论:上述中药组分配伍后能有效清除致癌有害物质多环芳烃;本实验采用的均匀设计结合荧光光谱检测方法,为筛选评价中药组分配伍清除致癌物多环芳烃的作用提供了合适的研究方法。
OBJECTIVE: To study the best compatibility effect of multiple carcinogens (PAHs) in vitro on the basis of uniform design method. Methods: Regression analysis with least angle regression (LARS) showed that the four Chinese medicinal components of total flavonoids of licorice, ginkgo biloba, total flavonoids of Herba Epimedii and Astragalus were clarified by fluorescence spectroscopy, Study on the Function of Aromatic Hydrocarbons. Results: The regression equations of compatibility of PAHs with PAHs were obtained by LRAS regression analysis. The optimized combination of PAHs was validated by pharmacodynamics. The results showed that the best combination of the ingredients of Chinese herbs was Glycyrrhiza Total Flavone 3.144 g · L-1 , Ginkgo biloba extract 0.8 g · L-1, epimedium flavone 0.024 g · L-1 and total astragalus 0.036 g · L-1. CONCLUSION: The above traditional Chinese medicine components can effectively remove the carcinogenic substance PAH after compatibility. The uniform design and fluorescence detection method used in this experiment provided a suitable research for screening and evaluating the role of traditional Chinese medicine components in eliminating carcinogenic PAHs method.