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目的检测小鼠胚胎造血发育中维甲酸(retinoic acid,RA)受体(RA receptors,RARs)不同亚型RAR-α、RAR-β、RAR-γ及其合成酶视黄醛脱氢酶1(retinal dehydrogenase 1,Raldh1)和Raldh2的转录水平,探讨RARs不同亚型及其合成酶在胚胎造血发育中的作用。方法获取孕鼠胎龄9.5d(embryonic day 9.5,E9.5)卵黄囊(yolk sac,YS),E10.5、E11.5主动脉-性腺-中肾(aorta-gonad-mesonephros,AGM)区,E13.5、E14.5、E17.5胎肝(fetal liver,FL)和成年小鼠骨髓(bone marrow,BM),运用实时荧光定量PCR检测各组织中RAR-α、RAR-β、RAR-γ、Raldh1和Raldh2的转录水平。结果与其他不同阶段造血组织比较,在E9.5 YS、E11.5 AGM区和BM中,RAR-α、RAR-β、RAR-γ及Raldh1、Raldh2均高表达(P<0.01)。E11.5 AGM区RAR-α、RAR-β、RAR-γ及Raldh1、Raldh2表达水平高于E10.5 AGM区(P<0.01)。而E13.5~E17.5FL间的RAR-β、RAR-γ及Raldh1、Raldh2表达水平差异无统计学意义。结论YS、AGM区和BM中RAR-α、RAR-β、RAR-γ及其合成酶Raldh1和Raldh2表达趋势与已有研究中相应造血组织来源的红系造血祖细胞改变相一致,反映了RA与小鼠个体发育中YS、AGM区和BM的造血形成密切相关,而RA对FL的造血形成作用可能并不显著。
Objective To detect the expression of RAR-α, RAR-β, RAR-γ and their synthase retinaldehyde dehydrogenase 1 (RARs) in different subtypes of retinoblastoma (RA) retinal dehydrogenase 1, Raldh1) and Raldh2 transcriptional levels to explore the role of different subtypes of RARs and their synthase in embryonic hematopoiesis. Methods The gestational day 9.5 (E9.5) yolk sac (YS), E10.5, E11.5 aorta-gonad-mesonephros (AGM) , E13.5, E14.5, E17.5 fetal liver (FL) and adult mouse bone marrow (BM) were detected by real-time fluorescent quantitative PCR. The expression of RAR-α, RAR- -γ, Raldh1 and Raldh2 transcription levels. Results Compared with other hematopoietic tissues of different stages, the expression of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 were highly expressed in E9.5 YS, E11.5 AGM and BM (P <0.01). The expression of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 in E11.5 AGM region was higher than that in E10.5 AGM region (P <0.01). There was no significant difference in the expression of RAR-β, RAR-γ, Raldh1 and Raldh2 between E13.5 and E17.5FL. CONCLUSIONS: The expression of RAR-α, RAR-β, RAR-β, RAR-γ, and their synthetic enzymes Raldh1 and Raldh2 in YS, AGM and BM are consistent with the changes of hematopoietic progenitor erythrocytic hematopoietic progenitor cells in the present study. It is closely related to the hematopoiesis of YS, AGM region and BM in mouse individual development, and the effect of RA on hematopoietic formation of FL may not be significant.