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研究一步层析制备高比活抑肽酶新工艺。方法：将化学改性与修饰壳聚糖体，共价偶联配基牛胰蛋白酶，制成固定化酶为抑肽酶亲和吸附剂，直接对黄牛肺提取液一步层析纯化，再超滤脱盐、浓缩、去热原，冻干即抑肽酶。结果：抑肽酶比活５７００ｋｉｕ／ｍｇ，收率２２×１０~4ｋｉｕ／ｋｇ（牛肺）；固定化酶单位活力２５９５０ｋｉｕ／ｇ（干），酶活性回收率５５％，非特异性吸附较小，机械强度高，可反复使用。结论：工艺过程简便，样品比活较高，质量稳定，成本低，适合工业化大生产。
Study of a new high-specific activity of aprotinin chromatography. Methods: Chemically modified chitosan and chitosan were covalently conjugated with alanyltransferase to prepare immobilized enzyme for aprotinin affinity adsorbent. Filter desalination, concentrated, to pyrogen, lyophilized that aprotinin. Results: The specific activity of aprotinin was 5700kiu / mg and the yield was 22 × 10 ~ 4kiu / kg (bovine lung). The activity of immobilized enzyme was 25950kiu / g (dry), the recovery of enzyme activity was 55% High mechanical strength, can be used repeatedly. Conclusion: The process is simple, high specific activity, stable quality, low cost, suitable for large-scale industrial production.