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目的研究儿童局灶性皮质发育不良I型(focal cortical dysplasia type I,FCDI)和IIa型(FCDIIa)所致的难治性癫痫病灶中瞬时感受器电位辣椒素受体1型(transient receptor potential vanilloid receptor 1,TRPV1)的表达,探讨其在FCD所致难治性癫痫发生机制中的作用。方法选取20例儿童FCDI型、FCDIIa型难治性癫痫患者临床手术切除的皮层致痫灶标本,进行FCD病理分型后与对照组(8例尸检来源的正常皮层标本)作比较,利用RT-PCR、Western Blot等技术检测FCDⅠ型和FCDⅡa型致痫灶中TRPV1的mRNA、蛋白水平的表达情况,并进一步利用免疫组织化学(IHC)研究TRPV1的细胞定位。结果在FCDI型、FCDIIa型致痫灶皮层中,RT-PCR与Western blot结果显示,TRPV1在mRNA、蛋白水平均较对照组明显升高(P<0.05)。IHC结果显示,在正常皮层中TRPV1在神经元和胶质样细胞上都有表达,而在FCD致痫灶中与癫痫发作程度密切相关的“柱形结构”和异型神经元上免疫染色强度明显高于正常皮层。结论 FCDI型、FCDIIa型难治性癫痫患者致痫灶皮层中TRPV1的表达增高,提示其可能与FCD所致难治性癫痫密切相关。
OBJECTIVE: To investigate the effects of transient receptor potential vanilloid receptor (PI) in focal refractory epilepsy induced by focal cortical dysplasia type I (FCDI) and type IIa (FCDIIa) 1, TRPV1) in the pathogenesis of FCD induced refractory epilepsy. Methods Twenty cases of FCDI-type and FCDIIa-type epilepsy in children with clinically resected cortical epileptogenic lesions were selected and compared with the control group (8 normal cadaver specimens from autopsy) after FCD pathological classification. RT- PCR and Western Blot were used to detect the mRNA and protein expression of TRPV1 in FCD type I and FCDIIa epileptogenic zones. The cellular localization of TRPV1 was further studied by immunohistochemistry (IHC). Results The mRNA and protein levels of TRPV1 in FCDI and FCDIIa epileptogenic cortex were significantly higher than those in control group (P <0.05) by RT-PCR and Western blot. The results of IHC showed that TRPV1 was expressed on both neuronal and glial-like cells in normal cortex and was immunostained on “columnar structure ” and abnormal neurons closely related to the degree of seizure in FCD epileptic foci Intensity was significantly higher than normal cortex. Conclusion The expression of TRPV1 in epileptogenic cortex of patients with FCDI and FCDIIa refractory epilepsy is increased, suggesting that it may be closely related to refractory epilepsy induced by FCD.