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为探讨环氧乙烷（Ｅｔｏ）对细胞内主要修饰酶ＤＮＡ甲基化酶活性的影响，对Ｗｉｓｔａｒ雄性大鼠采用静式吸入染毒，浓度７５１．７ｍｇ／ｍ３，每天２小时，每周６天，连续染毒９周。对其肝、肾和睾丸ＤＮＡ进行限制性内切酶同裂酶（ＭｂｏＩ和Ｓａｕ３ＡＩ，ＨｐａⅡ和ＭｓｐⅠ）敏感性试验和Ｓｏｕｔｈｅｒｎ印迹杂交分析。结果：大鼠肝、肾和睾丸ＤＮＡ经ＨｉｎｄⅢ、ＭｂｏⅠ和Ｓａｕ３ＡⅠ酶解后呈完全酶解带型，仅见１条杂交带。经ＨｐａⅡ和ＭｓｐⅠ酶解后呈不完全酶解带型，均见多条杂交带。电泳带型、杂交带条数及放射自显影强度在染毒组和对照组之间差异均无显著性。表明Ｅｔｏ对大鼠肝、肾、睾丸ＤＮＡ甲基化酶活性无影响，睾丸ＤＮＡ甲基化程度远低于肝和肾。
To investigate the influence of ethylene oxide (Eto) on DNA methyltransferase activity of major intracellular enzymes, Wistar male rats were treated with inhalation of static inhalation at a concentration of 751.7mg / m3, 2 hours a day, 6 Days, continuous exposure to 9 weeks. Restriction endonuclease (MboI and Sau3AI, HpaII and MspI) susceptibility assays and Southern blot hybridization analyzes were performed on their liver, kidney and testis DNA. RESULTS: The DNA of rat liver, kidney and testis was completely digested with Hind Ⅲ, Mbo Ⅰ and Sau3A Ⅰ, and only one hybridized band was found. After digestion with HpaⅡand MspⅠ, it was incompletely digested, showing multiple hybridization bands. Electrophoresis zone type, the number of hybridization bands and autoradiography intensity in the exposure group and the control group, no significant difference. The results showed that Eto had no effect on DNA methylase activity in liver, kidney and testis, and DNA methylation in testis was much lower than that in liver and kidney.