诊断黑热病最可靠的方法是检查病人体内有无病原体。但有时由于所得的材料含虫量较少 ,涂片检查往往为阴性 ,给诊断带来一定困难。为提高病原体检出率和诊断率 ,常采用体外培养法培养出前鞭毛体 ( promastigote)后再进行制片 ,染色。培养时一般多采用三 N( Nory- Mac Neal- Nic The most reliable way to diagnose kala-azar is to check for the presence or absence of pathogens in the patient’s body. But sometimes the resulting material contains less insects, smear tests are often negative, to the diagnosis of certain difficulties. In order to improve the detection rate and diagnosis rate of pathogens, promastigote cells are usually cultured in vitro and then prepared and stained. Cultivation is generally more than three N (Nory - Mac Neal - Nic