论文部分内容阅读
目的了解2015年昌平区副溶血性弧菌临床菌株耐药状况及分子特征。方法药敏实验按照美国临床实验室标准化协会(CLSI)推荐的K-B纸片法进行24种抗生素的敏感性分析;PFGE实验参照Pulse Net脉冲场凝胶电泳实验方法。结果药敏结果显示,副溶血性弧菌对阿米卡星和妥布霉素完全耐药,对萘替米星耐药率高达95.45%,对米诺环素和卡那霉素处于耐受和敏感之间,对氨苄西林和头孢呋新钠耐药或介于耐药和敏感之间。耐药谱分析显示,副溶血性弧菌同时耐受3~7种抗生素。PFGE分析发现,22株副溶血性弧菌有17种PFGE带型,可分为2个聚类群,血清型O3∶K6属于A群且大部分菌株对氨苄西林、头孢呋新钠耐药。结论昌平区引起腹泻的副溶血性弧菌耐受抗生素的种类多、多重耐药严重且PFGE带型呈高度多态性。
Objective To investigate the drug resistance and molecular characteristics of clinical strains of Vibrio parahaemolyticus in Changping District in 2015. Methods Susceptibility testing 24 antibiotics susceptibility assays were performed according to the K-B disk method recommended by the American Society for Clinical Laboratory Standards (CLSI); PFGE experiments were based on the Pulse Net pulsed-field gel electrophoresis assay. Results The results of drug susceptibility test showed that Vibrio parahaemolyticus was completely resistant to amikacin and tobramycin, the drug resistance rate to naltrexamine was as high as 95.45%, and was resistant to minocycline and kanamycin And sensitive to ampicillin and cefuroxime sodium resistance or resistance and sensitivity between. Resistance spectrum analysis showed that Vibrio parahaemolyticus resistant to 3 to 7 kinds of antibiotics at the same time. PFGE analysis showed that 22 strains of Vibrio parahaemolyticus had 17 PFGE patterns and could be divided into two clusters. Serotype O3: K6 belonged to group A and most of the strains were resistant to ampicillin and cefuroxime. Conclusion The strains of Vibrio parahaemolyticus that cause diarrhea in Changping district are more resistant to antibiotics, with more multi-drug resistance and highly polymorphic PFGE bands.