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目的:建立测定磷霉素氨丁三醇效价的方法。方法:采用浊度法测定磷霉素氨丁三醇含量,对药典规定的检定菌进行选择,并对微生物浊度法测定磷霉素氨丁三醇效价进行试验设计,结果与管碟法相比较。结果:选择大肠埃希菌为检定菌,浊度法线性范围为12～42u·mL-1,线性方程:Y=-0.7806log C+1.555,r=0.9983。结论:浊度法与管碟法的测定结果无显著性差异,可作为磷霉素氨丁三醇的效价测定方法之一,且更快捷、方便。
Objective: To establish a method for determining the potency of fosfomycin tromethamine. Methods: The fosfomycin trometamol content was determined by turbidimetric method, and the selected strains were selected by the pharmacopoeia. The titer of fosfomycin tromethamine was determined by the method of microbial turbidity test. Compare Results: The selection of Escherichia coli as test bacteria, turbidity linear range of 12 ~ 42u · mL-1, linear equation: Y = -0.7806log C + 1.555, r = 0.9983. Conclusion: There is no significant difference between the turbidimetric method and the tube plate method, which can be used as one of the methods to determine the titer of fosfomycin tromethamine, which is faster and more convenient.