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目的检测宫颈鳞癌组织及正常宫颈组织中HIF-2α、VEGF基因与蛋白的表达情况,探讨其在子宫颈鳞癌的临床意义。方法随机选取64例子宫颈鳞癌组织和22例正常宫颈组织,收集年龄、FIGO分期和淋巴结转移等临床相关指标,采用实时荧光定量PCR法及免疫组织化学法(Elivision法)检测各组织中HIF-2α、VEGF的表达。结果实时荧光定量PCR分析结果表明HIF-2α、VEGF mRNA表达较正常宫颈组织显著增加,差异有统计学意义(P<0.05),且二者mRNA水平呈正相关(r=0.778,P<0.001)。免疫组织化学法结果表明HIF-2α、VEGF蛋白在宫颈组织中的阳性表达率分别为宫颈鳞癌组93.8%、正常宫颈组18.2%,两组比较差异有统计学意义,且HIF-2α与VEGF呈显著相关(r=0.514,P<0.05)。HIF-2α、VEGF的mRNA表达均与年龄无关,但与FIGO分期、淋巴结转移关系密切(P<0.05),FIGO分期高、有淋巴结转移的子宫颈癌组织HIF-2α、VEGFmRNA表达水平均相应对照组升高。结论与正常宫颈组织相比,HIF-2α、VEGF mRNA和蛋白在宫颈鳞癌中均呈高表达。
Objective To detect the expression of HIF-2α and VEGF gene and protein in cervical squamous cell carcinoma and normal cervical tissue, and to investigate its clinical significance in cervical squamous cell carcinoma. Methods 64 cases of squamous cell carcinoma of the cervix and 22 cases of normal cervical tissue were randomly selected. The clinical, age, FIGO staging and lymph node metastasis were collected. The expression of HIF-1α in various tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry. 2α, VEGF expression. Results The results of real-time quantitative PCR showed that the mRNA expression of HIF-2α and VEGF were significantly increased compared with that of normal cervix (P <0.05), and the mRNA levels of HIF-2α and VEGF were positively correlated (r = 0.778, P <0.001). Immunohistochemical results showed that the positive rates of HIF-2α and VEGF in cervical tissue were 93.8% in cervical squamous cell carcinoma group and 18.2% in normal cervical group, respectively. The difference between the two groups was statistically significant, and the positive rates of HIF-2α and VEGF There was a significant correlation (r = 0.514, P <0.05). The mRNA expression of HIF-2α and VEGF was not related with age, but closely correlated with FIGO stage and lymph node metastasis (P <0.05). The expression level of HIF-2α and VEGF mRNA in cervical cancer with high FIGO stage and lymph node metastasis Group increased. Conclusion Compared with normal cervical tissue, HIF-2α and VEGF mRNA and protein are both highly expressed in cervical squamous cell carcinoma.