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应用北京医科大学培育的听源性癫痫易感大鼠Ｐ７７ＰＭＣ，以癫痫不易感大鼠Ｗｉｓｔａｒ为对照，系统研究了ＮＭＤＡ受体亚单位Ⅰ（ＮＲ１）与白细胞介素１（ＩＬ１）在癫痫发生发展中的作用及相互关系。实验在整体、脑片、神经细胞培养及分子水平进行。所得较有意义的结果如下：（１）在听源性惊厥易感大鼠Ｐ７７ＰＭＣ整个发育过程中脑内ＮＲ１ｍＲＮＡ的表达，以及成年鼠脑内ＮＭＤＡ受体活性（ＭＫ８０１结合）都高于对照组Ｗｉｓｔａｒ大鼠。惊厥后Ｐ７７ＰＭＣ脑内ＮＲ１ｍＲＮＡ的表达呈时间依赖性增加，惊厥后２４ｈ比惊厥前增加１１１％～２０２％；ＮＲ１反义寡核苷酸脑室注射（每μｌ１０μｇ）可显著减轻Ｐ７７ＰＭＣ大鼠惊厥程度，并可对谷氨酸引起的体外神经细胞的损伤有保护作用，抑制谷氨酸导致的神经毒性作用。由此证实ＮＲ１参与惊厥的发生发展，并与Ｐ７７ＰＭＣ大鼠的遗传性癫痫易感性关系密切。（２）在神经细胞培养中ＩＬ１β可明显剂量依赖性地（１～２５Ｕ·ｍｌ１）提高ＮＲ１ｍＲＮＡ的表达，白细胞介素１受体拮抗剂（ＩＬ１ｒａ）可阻断此效应；ＩＬ１β可剂量依赖性地提高ＮＲ１受体活性，因此提示ＩＬ１具有兴奋性神经调质的作用，其作?
Application of Beijing Medical University to cultivate the auditory epilepsy susceptible rat P77PMC to epilepsy in rats Wistar as a control, the system of NMDA receptor subunit Ⅰ (NR 1) and interleukin 1 (IL 1 ) In the development of epilepsy and their relationship. Experiment in the whole, brain slices, nerve cell culture and molecular level. The more meaningful results obtained are as follows: (1) In the whole process of development of auditory convulsion-prone P77PMC, the expression of NR-1 mRNA in the brain and the NMDA receptor activity (MK-801 binding) in adult rat brain were both high Wistar rats in control group. The expression of NR-1 mRNA in the brain of P77PMC increased in a time-dependent manner after convulsion, and increased by 111% ~ 202% at 24h after convulsion. Compared with preconditioning, NR1 antisense oligonucleotide injected intracerebroventricularly (10μg / μl) significantly reduced P77PMC rats Convulsions, and glutamate-induced damage to nerve cells in vitro protective effect of glutamate-induced neurotoxicity. Thus confirmed that NR 1 involved in the occurrence and development of convulsions and P77PMC rats with genetic epilepsy susceptibility are closely related. (2) In the neural cell culture IL 1β significantly increased dose-dependently (1 ~ 25U · ml 1) NR 1mRNA expression, interleukin 1 receptor antagonist (IL 1ra) can be blocked This effect; IL 1β dose-dependent increase in NR 1 receptor activity, suggesting that IL 1 excitatory neuromodulation effect, which?