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以小麦光温敏核雄性不育系BS20×Fu3双单倍体(DH)群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁群(除4D、6A),不同连锁群标记数为2~15个,长度在15.3~244.4 c M之间,平均长度为144.7 c M,标记之间平均遗传距离为17.4 c M。同时构建3个DNA池(包括恢复池、北京不育池和阜阳不育池),用分离群体分组分析法(BSA)对育性进行分析,筛选出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法(MCIM)对育性进行QTL分析,当F>7.5时,检测到6个主效QTL,用复合区间作图法(CIM)对育性进行QTL分析,当LOD值>2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果,位于1BL、2BS和3AL上的小麦光温敏不育基因是真实的。
A total of 243 pairs of polymorphic primers were screened from 1112 pairs of SSR and EST-SSR primers using 289 lines of wheat double thermo-sensitive genic male sterile line BS20 × Fu3 double haploid (DH) population. Using 128 SSR markers and six EST-SSR markers were used to construct a genetic linkage map. The map covered 2749.2 cM in 19 linkage groups (except 4D and 6A), 2 to 15 markers in different linkage groups, Between 15.3 and 244.4 cM, the average length was 144.7 cM, and the average genetic distance between markers was 17.4 cM. At the same time, three DNA pools (including recovery pool, Beijing sterile pool and Fuyang sterile pool) were constructed. Fertility was analyzed by segregating population analysis (BSA). The selected polymorphic primers were Wmc264, Wmc73 and Xgwm350 , Distributed in 3A, 5B, 2A / 7D chromosomes. At the same time, QTL analysis of fertility was carried out by mixed linear interval mapping (MCIM). When F> 7.5, six major QTLs were detected and QTL analysis of fertility was performed by composite interval mapping (CIM) Thirteen main-effect QTLs were detected when the LOD value was> 2.5. Three QTLs were detected by the two methods, which were 1BL Wmc365-cfa2129, 2BS Wmc602-Xgwm148 and 3AL Wmc264a-cfa2262, respectively. Based on the results of BSA and QTL, wheat photo-thermo-sensitive genic genes located on 1BL, 2BS and 3AL were true.