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为观察深低温保存角膜行穿透性移植治疗角膜溃疡穿孔的效果，应用深低温保存的人尸角膜行穿透性移植治疗角膜溃疡穿孔３５例（３５眼）。经６～２３个月的随访，３４眼的角膜穿孔均被修复，并控制了感染。２０眼术后植片保持透明，９眼半透明，５眼混浊，１眼单疱性角膜炎复发再次穿孔后失明；２９眼视力有不同程度改善。重症虹膜炎（１７．１％）、排斥反应（１７．１％）和继发青光眼（１４．３％）是主要术后合并症和植片失败原因。结果表明，深低温保存角膜穿透性移植是治疗角膜溃疡穿孔的有效方法。
To observe the effect of cryogenic preservation of corneal perforation on perforation of corneal ulcer, percutaneous transplanting of corneal ulcer with cryopreservation was performed in 35 cases (35 eyes). After 6 to 23 months of follow-up, 34 eyes of corneal perforation were repaired and control of the infection. Postoperatively, 20 eyes remained clear, 9 eyes were semitransparent, 5 eyes were cloudy, 1 eyes had unilateral puncture keratitis, and blindness was found after perforation; 29 eyes had some degree of vision improvement. Severe iritis (17.1%), rejection (17.1%), and secondary glaucoma (14.3%) were the major causes of postoperative complications and graft failure. The results show that cryopreservation of corneal penetrating transplantation is an effective method for the treatment of corneal ulcer perforation.