本文主要论述细胞膜表面抗原的单克隆抗体。细胞膜表面抗原的识别之所以重要,原因有两方面:①通过膜抗原可对活细胞进行亚型划分,以便进行生物学研究;②细胞膜表面抗原是肿瘤特异性治疗的重要靶子。已发现,现有抗肿瘤组织的抗体多数能识别各种来源于上皮的肿瘤和正常上皮细胞的表面抗原,少数抗体则似能鉴别与某个种系有关的抗原。根据各种鳞状细胞癌抗体中极少数抗体的研究,提示很可能有一种抗原在鳞癌有鉴别性表达,而在肺腺癌上无此表现。与此相反,有证据表明,有些抗原在腺癌细胞上选择性表达,而在鳞癌上却不表达。最近,作者发表了关于间皮细胞膜抗原抗体的报道,这种抗体能区分间皮和腺癌。实际上,所有这些 NSCLC 的抗体也能与相应的正常组织相结合,如支气管、肺泡内膜细胞或正常间皮细胞。但这并不排除肿瘤和正常组织有表达的差别,因为当所用的方法检测阈值很低时(如采用免疫过氧化物酶染色),无法观察到抗原密度方 This article discusses the monoclonal antibody to cell surface antigen. The reason why the identification of cell surface antigen is important for two reasons: (1) the subtype of living cells can be divided by membrane antigen for biological research; (2) cell surface antigen is an important target of tumor-specific treatment. It has been found that most of the existing anti-tumor tissue antibodies recognize a variety of surface antigens derived from epithelial tumors and normal epithelial cells. A few antibodies appear to be capable of identifying antigens associated with a certain species. According to a variety of squamous cell carcinoma antibodies in a very small number of antibodies, suggesting that there is likely to be an antigen in squamous cell carcinoma was differentially expressed, but not in lung adenocarcinoma. In contrast, there is evidence that some of the antigens are selectively expressed on adenocarcinoma cells but not on squamous cell carcinomas. Recently, the authors published a report on mesothelial cell membrane antigen antibodies that discriminate between mesothelium and adenocarcinoma. In fact, all of these NSCLC antibodies can also be associated with the corresponding normal tissue, such as bronchial, alveolar endometrial cells or normal mesothelial cells. However, this does not rule out the difference in expression between tumor and normal tissue because the antigen density can not be observed when the method used has a low detection threshold (eg, immunoperoxidase staining)