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目的利用基于RNA-seq的二代测序技术分析日本血吸虫侵袭前后的湖北钉螺转录组的表达,为进一步完善湖北钉螺基因结构信息及挖掘血吸虫感染钉螺相关分子标记提供实验数据。方法分别提取日本血吸虫毛蚴感染后第7天、第30天湖北钉螺组织和正常钉螺组织的总RNA,经RNA质量检测合格后建立e DNA文库,利用Illumina NextSeq500测序仪进行测序得到原始序列数据;测序数据经过滤和de novo拼接后,进一步开展转录本注释和聚类分析。结果共得到63 686条广泛通用的基因数据(Unique gene,Unigene),基因功能注释及功能群组聚类分析结果显示,一般性功能蛋白质编码Unigene占比最多(15.36%),其次是信号转导(11.75%)、转录后修饰(8.89%)等,此外还有较高比例的未知功能蛋白质编码Unigene(12.20%)。结论日本血吸虫侵袭后的湖北钉螺转录组表达信息显示有数个基因呈显著性上调或下调表达,本研究为从分子水平研究湖北钉螺与血吸虫感染相互作用奠定了基础。
OBJECTIVE: To analyze the expression of the snail transcriptome of Schistosoma japonicum before and after the invasion by Schistosoma japonicum using RNA-seq-based second-generation sequencing technology and to provide experimental data for further improvement of snail gene structure information and the detection of related snail snail infection markers in Schistosoma japonicum. Methods The total RNA was extracted from the tissues of Oncomelania hupensis and normal snails on the 7th and the 30th days after infection with the miracidia of Schistosoma japonicum respectively. The original DNA was sequenced after sequenced according to the quality control of the RNA. Sequencing was performed using the Illumina NextSeq500 sequencer. Sequencing After data filtering and de novo splicing, further transcript annotation and cluster analysis were performed. Results A total of 63 686 unique gene (Unigene) genes were obtained. Gene functional annotation and functional group clustering analysis showed that most of the functional proteins were Unigene (15.36%), followed by signal transduction (11.75%), post-transcriptional modification (8.89%), etc. In addition, there is a higher proportion of unknown function protein encoding Unigene (12.20%). Conclusion The expression information of S. japonicum transcriptome after invaded Schistosoma japonicum shows that several genes were significantly up-regulated or down-regulated. This study laid the foundation for the study on the interaction between S. japonicum and schistosoma infection at the molecular level.