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本文通过建立大鼠桡骨骨折生理性模型,探讨了紫芝子实体不同极性提取物促进骨折愈合的作用。首先制备紫芝的石油醚、乙酸乙酯、乙醇、水等提取物,对选取的144只雌雄各半大鼠制作桡骨骨折模型,之后随机分为8组,每组分别给紫芝不同极性的提取物,并于连续灌胃10d、20d、30d用X射线仪和光学显微镜观察各给药组大鼠的骨痂组织生成和大鼠脏器及骨折部位的病理组织学改变情况,检测大鼠血清中钙、磷、碱性磷酸酶含量。结果表明,影像学观察后紫芝不同提取物对大鼠骨折具有不同程度的促进愈合作用,其中,水煎组显示出明显的促进愈合作用,于30d时骨痂已形成,骨性愈合,骨折线消失,骨髓腔复原,骨折已经基本痊愈,水提取物组其次,而其他3组与模型对照组骨折大鼠愈合时间相近。病理组织学观察表明水提取物组和水煎组对大鼠脾、胸腺、肝、肾脏各脏器无任何影响。对骨组织切片染色后观察,灌胃10d后可见造模的7组大鼠骨折边缘均出现大量间充质细胞,而水煎组出现软骨连接;于20d观察两种水提物组骨折边缘间充质细胞退化,少量胶原纤维包绕骨折骨,断骨近端软骨细胞退化,骨小梁相互连接向板层骨转变,而其余各组均形成软骨连接;于30d观察发现7组骨折边缘间充质细胞均出现退化,而水煎组骨折处胶原纤维与正常骨膜无明显差异。对钙含量的检测中,水煎提取物组与模型对照组比较,具有极显著差异(P<0.01);对磷含量的检测中,石油醚、乙酸乙酯、水煎提取物组与模型对照组比较有极显著差异(P<0.01);对碱性磷酸酶含量的检测中,水煎组与阳性对照组、与模型对照组相比均呈上升趋势。结果表明紫芝子实体水煎组具有较好地促进大鼠桡骨骨折的愈合作用。
In this paper, we established a physiological model of radial fractures in rats and explored the role of different polarity extracts of Fructus Tsorachis to promote fracture healing. Firstly, the petroleum ether, ethyl acetate, ethanol, water and other extracts of Zizyphus jujuba were prepared. Radial fracture models were made to 144 selected male and female rats, which were then randomly divided into 8 groups. Each group was given different polarities The rats were sacrificed and the rats were sacrificed at the end of the experiment. The rats were sacrificed at 10, 20 and 30 days after gavage. The pathological changes of the callus tissue and the organs and fractures of the rats were observed by X-ray and optical microscopy. In calcium, phosphorus, alkaline phosphatase content. The results showed that different extracts of Ganoderma lucidum could promote the healing of rat fracture in different degree after the imaging observation. Among them, the decoction group showed obvious promoting healing effect. At the 30th day, the callus was formed, the bony union, fracture line Disappeared, bone marrow cavity recovery, fracture has basically recovered, followed by water extract group, while the other three groups and the model control group fracture healing time similar. Histopathological examination showed that water extract group and decoction group had no effect on the organs of spleen, thymus, liver and kidney in rats. The bone tissue sections were stained and observed. After 10 days of intragastric administration, a large number of mesenchymal cells appeared on the margin of the fracture in the 7 groups of rats, while cartilage was connected in the decoction group. At 20 days, A small amount of collagenous fibers surrounded the fractured bone, degenerated cartilage cells in the proximal part of fractured bone, and trabecular meshwork connected to the lamina propria, while the rest of the groups formed cartilage connections. At 30 days, seven groups of fractured margins Mesenchymal cells were degenerated, but there was no significant difference between collagen fibers and normal periosteum in the decoction group. In the determination of calcium content, there was a significant difference (P <0.01) between the decoction extract group and the model control group. In the detection of phosphorus content, petroleum ether, ethyl acetate, decoction extract group and model control (P <0.01). In the detection of alkaline phosphatase, both the decoction group and the positive control group showed an upward trend compared with the model control group. The results showed that fructus cnidii decoction group has better promote the healing of radial fractures in rats.