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与裸碳糊电极相比,胭脂红与dsDNA的嵌插作用在TiO_2掺杂的碳糊电极上得到增强.这是由于纳米TiO_2的存在,增加了胭脂红的电子传递速率.基于胭脂红与dsDNA的强嵌插作用,当dsDNA的浓度在21.24~127.44μg·mL~(-1)时,胭脂红的氧化还原峰电流下降值与dsDNA的浓度成正比,检测限为16.04μg·mL~(-1).而在裸电极上,dsDNA的浓度范围为31.76~74.34μg·mL~(-1),检测限只有30.32μg·mL~(-1).对于这两种电极,胭脂红与dsDNA的结合常数分别是4.92×10~8,6.41×10~7L·mol~(-1),结合比均为1∶2.因此,胭脂红与dsDNA在纳米TiO_2掺杂的碳糊电极嵌插作用被用于测定合成样品中的dsDNA,平均回收率在96.02%~102.5%.
Compared with bare carbon paste electrode, the intercalation of carmine and dsDNA was enhanced on the TiO_2 doped carbon paste electrode, which increased the carmine electron transport rate due to the presence of nano-TiO 2. When the concentration of dsDNA was between 21.24 and 127.44μg · mL -1, the decrease of redox peak current of carmine was proportional to the concentration of dsDNA, the detection limit was 16.04μg · mL ~ (-1) 1), while the concentration of dsDNA on the bare electrode ranged from 31.76 to 74.34μg · mL -1 with a limit of detection of only 30.32μg · mL -1 For both electrodes, the ratio of carmin and dsDNA The binding constants were 4.92 × 10 ~ 8, 6.41 × 10 ~ 7L · mol ~ (-1), respectively, and the binding ratios were all 1: 2. Therefore, carmine and dsDNA were intercalated into nano-TiO_2 carbon paste electrode For the determination of dsDNA in synthetic samples, the average recoveries ranged from 96.02% to 102.5%.