论文部分内容阅读
目的 探讨慈菇对镉致大鼠急性肝损伤的保护作用及其机制。方法 36只雄性SD大鼠随机分为6组（n=6）,分别用25％、50％、75％的慈菇（2ml/100g体重）,维生素E（50mg/100g体重）及等量的蒸馏水灌胃,连续10天,末次灌胃24h后,腹腔注射CdCl2（20μmol/kg体重）,染毒24h后,比较各组血清乳酸脱氢酶（LDH）、谷丙转氨酶（GPT）活性,肝组织谷胱甘肽（GSH）、丙二醛（MDA）、镉（Cd）含量及超氧化物歧化酶（SOD）、谷胱苷肽过氧化物酶（GSH-PX）活性。结果 中、高浓度慈菇均能降低血清LDH、GPT活性（P<0.05）,降低肝组织MDA含量（P<0.05）,提高肝组织GSH含量（P<0.05）;高浓度慈菇组还降低了肝组织镉含量（P<0.05）;而VE仅降低了肝组织MDA含量（P<0.05）。结论 预先给予慈菇对镉致大鼠的急性肝损伤具有一定保护作用,且效果优于VE,其机制可能是同时增强了抗氧化和解毒的两个过程。
Objective To investigate the protective effect and mechanism of Sagitta cirrhosa on cadmium-induced acute liver injury in rats. Methods Thirty-six male Sprague-Dawley rats were randomly divided into 6 groups (n=6), used 25%, 50%, 75% Sagittaria (2ml/100g body weight), vitamin E (50mg/100g body weight) and the same amount. Distilled water was intragastrically administered for 10 consecutive days. After the last intragastric administration for 24 hours, CdCl2 (20 μmol/kg body weight) was intraperitoneally injected. After exposure to the virus for 24 hours, serum lactate dehydrogenase (LDH) and alanine aminotransferase (GPT) activities were compared. Tissue glutathione (GSH), malondialdehyde (MDA), cadmium (Cd) content and superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) activity. Results Medium and high concentrations of Sagittaria can reduce serum LDH, GPT activity (P<0.05), reduce liver tissue MDA content (P<0.05), increase liver GSH content (P<0.05); The content of cadmium in liver tissue (P<0.05); while VE only decreased the content of MDA in liver tissue (P<0.05). Conclusion Pre-administration of Sagittaria sargentii has a protective effect on cadmium-induced acute liver injury in rats, and the effect is better than that of VE. The mechanism may be that the two processes of antioxidant and detoxification are enhanced at the same time.