论文部分内容阅读
用 Bam HI、Eco RI、Dra I、Hinf I、Hind III、Hpa II、Msp I、Pst I、Sau 3AI、Sma I、Xba I、Xho I和 Sal I等十三种限制性内切酶对方正银鲫(Carassius aur-atus gibelio)、白鲫(Carassius auratus cuvieri)和鲫(Carassius auratus auratus)的线粒体 DNA(mt DNA)进行单酶酶切以及其中八种识别六碱基对序列的限制性内切酶的双酶酶切,经琼脂糖凝胶电泳后,分析且计算出各酶切片断大小,得出三种鲫鱼的 mt DNA分子大小:方正银鲫为 15990±90碱基对(bp);白鲫为 16600±130碱基对(bP);鲫为 15540±140碱基对(bp),并且分别建立了方正银鲫、白鲫和鲫 mt DNA由 Bam HI、Pst I、Eco RI 及 Xba I等四种限制性内切酶构建的酶切图谱。
Thirteen restriction enzymes, Bam HI, Eco RI, Dra I, Hinf I, Hind III, Hpa II, Msp I, Pst I, Sau 3AI, Sma I, Xba I, Xho I and Sal I Mitochondrial DNA (mt DNA) from Carassius aur-atus gibelio, Carassius auratus cuvieri and Carassius auratus auratus were digested with single enzymes and within eight restriction sequences that recognize six base pair sequences Cut enzyme digested by two enzymes, after agarose gel electrophoresis, analysis and calculate the size of each digestion fragment, obtained three kinds of crucian carp mt DNA molecule size: Founder silver crucian carp was 15990 ± 90 bp (bp) ; Crucian carp was 16600 ± 130 base pairs (bP); crucian carp was 15540 ± 140 bp, and the mt DNA of Founder silver carp, white carp and crucian carp were determined by Bam HI, Pst I, Eco RI and Xba I and other four restriction enzymes constructed digestion map.