论文部分内容阅读
[目的]分别以甘薯品种徐薯22的叶片与茎尖作为外植体,研究利用不同外植体通过体细胞胚胎再生途径得到再生植株的方法。[方法]将徐薯22的叶片和茎尖分别置于MSB和MSD培养基中诱导胚性愈伤,再将胚性愈伤置于MS培养基培养,观察体细胞胚的发生情况,最后对不同外植体得到的植株再生频率进行比较。[结果]用叶片作为外植体得到的胚性愈伤平均诱导频率为95.69%,而茎尖的则为30.56%;不同外植体在体细胞胚发生途径中的形态特征有一定差异;用叶片作为外植体的植株再生频率为60.61%,用茎尖的则为22.00%,且采用不同外植体诱导得到的再生植株无形态变化。[结论]在该试验中,体细胞胚的发生及植株再生的最适外植体为甘薯品种徐薯22的叶片。
[Objective] The study aimed to study the method of regenerating plants by somatic embryo regeneration using different explants with leaves and shoot tips of sweet potato variety Xushu 22 as explants. [Method] The leaves and shoot tips of Xushu 22 were placed in MSB and MSD medium to induce embryogenic callus. Embryogenic callus was placed in MS medium to observe the occurrence of somatic embryos. Finally, The frequency of plant regeneration from different explants was compared. [Result] The average induction frequency of embryogenic callus with leaves as explant was 95.69% and that of shoot tip was 30.56%. The morphological characteristics of different explants in somatic embryogenesis were different. The frequency of plant regeneration was 60.61% for leaf explants and 22.00% for shoots, and no morphological changes were observed in regenerated plants induced by different explants. [Conclusion] In this experiment, the most suitable explant for the occurrence of somatic embryogenesis and plant regeneration was the leaf of sweet potato variety Xushu 22.