有关血小板计数的方法学探讨国内已有不少报道,多是在传统的稀释液的基础上进行改革的。血小板直接计数的方法将近30余种之多。本文仅就我院多年来应用血涂片方法做血小板计数,与直接计数方法做一对比资料介绍。方法一.毛细血管穿刺取血,第一滴血弃去,取第二滴血以30～45°角在玻片上向前推去,用力要均匀。直到血液推尽为止。推片时切忌用力,否则细胞可变形,分布不均匀。二.干燥后Wright染色。染色时间要适当,勿太浅,以免使血小板着色浅而影响计数。三.放大1000倍,计数在每个视野中的血小板 Methodology on Platelet Counting There have been many reports in China, mostly on the basis of the traditional dilution of the reform. The method of direct platelet count nearly 30 kinds of as much. This article only on our hospital blood smear method for many years to do platelet count, and direct counting method to do a comparative information. Method 1. Capillary puncture blood, the first drop of blood to discard, take the second drop of blood to 30 ~ 45 ° angle on the slide to push forward, forced to be uniform. Until the blood pushed out. Do not push the thrust plate, or cell deformation, uneven distribution. Second, Wright staining after drying. Dyeing time should be appropriate, not too light, so as not to light platelet count and affect the count. Third, zoom in 1000 times and count the platelets in each field of view