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AIM:To evaluate the relationship between the expression oflipopolysaccharides(LPS)binding protein(LBP)and CD14mRNA and the severity of liver injury in alcohol-fed rats.METHODS:Twenty Wistar rats were divided into twogroups:ethanol-fed group(group E)and control group(group C).Group E was fed with ethanol(5-12g.kg~(-1).d~(-1))and group C received dextrose instead of ethanol.Rats ofthe two groups were sacrificed at 4 weeks and 8 weeks.Levels of endotoxin and alanine transaminase(ALT)inblood were measured,and liver pathology was observedunder light and electronic microscopy.Expressions of LBPand CD14 mRNA In liver tlssues were determined by RT-PCRanalysis.RESULTS:Plasma endotoxln levels were increased moresignificantly in group E(129±21 )ng.L~(-1) and(187±35)ng·L~(-1) at 4 and 8 wk than in control rats(48±9) ng·L~(-1) and(53±11)ng·L~(-1),respectively(P<0.05).Mean values ofplasma ALT levels were(1867±250)nkat·L~(-1) and(2450±367) nkat.L~(-1) in Group E.The values were increased moredramatically In ethanol-fed rats than in Group C after 4 and 8weeds.In liver section from ethanol-fed rats,there weremarked pathological changes(steatosis,cell infiltration andnecrosis).In ethanol-fed rats,ethanol administration led toa significant increase in LBP and CD14 mRNA levelscompared with the control group(P<0.05).CONCLUSION:Ethanol administration led to a significantIncrease in endotoxin levels in serum and LBP and CD14mRNA expressions in liver tissues.The increase of LBP andCD14 mRNA expression might wake the liver more sensitiveto endotoxin and liver injury.
AIM: To evaluate the relationship between the expression oflipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats. METHODS: Twenty Wistar rats were divided into twogroups: ethanol- fed group (group E) and control group (group C) .Group E was fed with ethanol (5-12g.kg -1 (-1)) and group C received dextrose instead of ethanol.Rats of the two groups were sacrificed at 4 Weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tlssues were determined by RT-PCR analysis .RESULTS: Plasma endotoxln levels were increased moresignificantly in group E (129 ± 21) ng.L -1 and (187 ± 35) ng · L -1 at 4 and 8 wk than in control rats (48 ± 9) ng · L -1 (18 ± 250) nkat · L -1 and (2450 ± 367) -1, respectively, and (53 ± 11) ng · L -1, respectively nkat.L ~ (-1) in Group E.The values were increased mo redramatically In ethanol-fed rats than in Group C after 4 and 8 weeks in liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis) .In ethanol-fed rats, ethanol administration led toa significant increase in LBP and CD14 mRNA levels werecompared with the control group (P <0.05) .CONCLUSION: Ethanol administration led to a significantIncrease in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP andCD14 mRNA expression might wake the liver more sensitiveto endotoxin and liver injury.