本文报告一种用可溶性人精子蛋白作为包被抗原的抗精子抗体检测药盒。用ELISA技术检测9种不同抗原特异性单克隆抗体和3种不同种抗人精子抗体,证明本药盒的组织特异性和种特异性均较好,测定间和室间重复性也较满意。临床实践结果表明本药盒较常规检验方法方便、灵敏,特别适于基层推广使用. This article reports an anti-sperm antibody test kit that uses soluble human sperm protein as a coating antigen. Nine different antigen-specific monoclonal antibodies and three different kinds of anti-human sperm antibodies were detected by ELISA. The tissue specificity and species specificity of this kit were good, and the reproducibility between test and room was also satisfactory. The clinical practice results show that the kit is more convenient and sensitive than the routine test methods, especially for grassroots promotion and use.