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目的 探讨超声破坏微泡造影剂后对β-半乳糖苷酶报告基因在人肝癌细胞(QGY)中转染的影响。方法 将培养QGY细胞转于2 4孔培养板后,分为5组,分别为单纯加入质粒组(D) ;质粒+微泡组(D +M) ;质粒+超声组(D +U) ;质粒+超声+微泡组(D +U +M) ;脂质体+质粒组(L +D) ;进行β-半乳糖苷酶质粒DNA的转染。2d后,进行β-半乳糖苷酶染色,测定各组细胞转染率。结果 单纯质粒组细胞转染率为4.92 % ;质粒+微泡组转染率为6 742 % ;质粒+超声组转染率为2 9.73 % ;质粒+超声+微泡组转染率为5 0 .88% ;脂质体+质粒组转染率为12 .15 % ;结论 超声破坏微泡造影剂可促进报告基因β-半乳糖苷酶质粒在QGY细胞的转染,为肿瘤基因治疗提供一种新型基因转移系统。
Objective To investigate the effect of ultrasound on the transfection of β-galactosidase reporter gene in human hepatoma cells (QGY) after the microbubble contrast agent was destroyed by ultrasound. Methods The cultured QGY cells were transferred into 24-well plates and divided into 5 groups: plasmid group (D), plasmid + microbubble group (D + M), plasmid + ultrasound group (D + U) Plasmid + ultrasound + micro-bubble group (D + U + M); liposome + plasmid group (L + D); transfection of plasmid DNA of β-galactosidase. After 2 days, β-galactosidase staining was performed and the transfection efficiency of each group was determined. Results The plasmid transfection efficiency of plasmid group was 4.92%, that of plasmid + microbubble group was 6 742%, that of plasmid + ultrasound group was 2 9.73%, and that of plasmid + ultrasound + microbubble group was 50 .88%. The transfection rate of liposome + plasmid group was 12.15% .Conclusions Ultrasound-damaged microbubble contrast media can promote the transfection of reporter gene β-galactosidase plasmid in QGY cells and provide a New gene transfer system.