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目的:探讨过氧化物酶体增殖物激活受体γ(PPARγ)及中药清胰汤对急性胰腺炎相关性肺损伤(APALI)的干预作用。方法:选用健康成年雄性Wistar大鼠60只,随机分为6组:假手术组、模型组、PPARγ配体罗格列酮(ROSI)处理组、PPARγ拮抗剂GW9662处理组、中药清胰汤处理组、PPARγ拮抗剂GW9662+中药清胰汤处理组,每组10只。采用胆胰管逆行注入1.5%的脱氧胆酸钠(0.1m L/100g)建立APALI模型,造模成功后给予药物干预,于给药6h后处死动物,测定动脉血气PCO2、PO2含量,观察肺组织病理学改变。结果:(1)模型组与假手术组相比,PCO2明显升高,PO2明显下降(P<0.05),肺组织出现充血、水肿、中性粒细胞浸润及灶性肺不张等改变。(2)PPARγ配体ROSI组、中药清胰汤处理组与模型组相比,PCO2明显下降,PO2明显回升(P<0.05),肺组织病理改变明显减轻。(3)PPARγ拮抗剂GW9662处理组与模型组相比,PO2、PCd含量均无显著性差异(P>0.05),肺组织病理改变亦无明显变化。(4)PPARγ拮抗剂GW9662+中药清胰汤处理组与中药清胰汤处理组相比,PCO2有所升高,PO2有所下降(P<0.05),肺组织病理改变加重。结论:(1)PPARγ配体激活PPARγ后,可使APALI模型大鼠精神活动和呼吸情况明显改善,肺组织病理损伤明显减轻,且上述效应可被PPARγ拮抗剂所逆转,表明PPARγ及其配体对APALI具有保护作用。(2)清胰汤是治疗APALI的有效方剂,其作用机制可能是通过直接或间接激活PPARγ,起到配体激动剂的作用。
Objective: To investigate the effects of peroxisome proliferator - activated receptor γ (PPARγ) and Chinese herb Qingyi Decoction on acute pancreatitis - associated lung injury (APALI). Methods: Sixty healthy adult male Wistar rats were randomly divided into 6 groups: sham operation group, model group, PPARγ ligand rosiglitazone (ROSI) treatment group, PPARγ antagonist GW9662 treatment group, Qingyitang treatment group Group, PPARγ antagonist GW9662 + Qingyitang treatment group, 10 rats in each group. APALI model was established by retrograde injection of 1.5% sodium deoxycholate (0.1m L / 100g) into the biliary and pancreatic ducts. After the model was established successfully, the animals were sacrificed and animals were sacrificed 6h later. The contents of PCO2 and PO2 in the arterial blood gas were measured. Histopathological changes. Results: (1) Compared with sham operation group, PCO2 was significantly increased and PO2 was significantly decreased (P <0.05). There was congestion, edema, neutrophil infiltration and focal atelectasis in lung tissue. (2) Compared with the model group, PCO2 significantly decreased and PO2 significantly increased (P <0.05). The pathological changes of lung tissue were significantly reduced. (3) Compared with the model group, there was no significant difference in PO2 and PCd content in the PPARγ antagonist GW9662 treatment group (P> 0.05), and there was no significant change in the lung pathological changes. (4) Compared with Qingyitang treatment group, PCO2 increased, PO2 decreased (P <0.05), and pathological changes of lung increased. (1) PPARγ ligand activation of PPARγ can significantly improve the mental activity and respiration of rats with APALI model, and significantly reduce the pathological damage of lung tissue, and the above effects can be reversed by PPARγ antagonist, indicating that PPARγ and its ligand APALI has a protective effect. (2) Qingyi Decoction is an effective prescription for the treatment of APALI, which may play a role as a ligand agonist by directly or indirectly activating PPARγ.