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细菌视紫红质(BR)是嗜盐菌质膜上的一种跨膜蛋白质,具有独特的光学响应,与合成高分子材料的复合有望提供一种信息功能材料.制备了第96位天冬氨酸被缬氨酸取代的BR突变体BR-D96V及其高分子复合膜,并进行了相关基础研究.通过基因工程方法,在本身不表达BR的嗜盐菌L33中实现了新突变体BR-D96V的表达.尽管缬氨酸侧链具有强疏水性,取代天冬氨酸后的蛋白质仍然具有光学响应,但与野生型BR相比,中性的盐溶液环境中突变体BR-D96V的M态寿命延长近两个数量级,有利于未来作为信息材料使用.将此突变体蛋白质与合成高分子聚乙烯醇(PVA)均匀混合后制备得到蛋白质/聚合物复合功能膜,M态寿命比液体条件下又有显著的延长.还发现BR-D96V中间态的寿命对于体系的含水量具有较高的敏感性.
Bacteriorhodopsin (BR), a transmembrane protein on halophilic membrane, has a unique optical response and is expected to provide an information functional material in combination with synthetic polymeric materials. Aspartate 96 BR mutant BR-D96V and its polymer composite membrane with valine substitution were studied and related basic research was carried out.A new mutant BR- D96V Although the side chain of valine has strong hydrophobicity and the protein substituted for aspartic acid still has an optical response, compared with the wild-type BR, the mutant BR-D96V M State life prolonged by nearly two orders of magnitude, is conducive to the future as an information material to use this mutant protein and synthetic polymer polyvinyl alcohol (PVA) uniformly mixed to prepare a protein / polymer composite functional membrane, M state life than the liquid conditions Under another significant extension.It was also found that BR-D96V intermediate state of life for the system has a high water content sensitivity.