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目的探讨脆性组氨酸三联体(FHIT)和p53蛋白在结、直肠癌中的表达及临床病理学意义。方法采用EnVision两步法检测76例结、直肠癌和癌周黏膜组织及30例腺瘤标本中的FHIT和p53蛋白表达。结果FHIT在结、直肠腺癌中表达降低或缺失占51.3%,在癌组织学分级中高分化占10%,与中分化56%或低分化62.5%相比差异显著(P<0.05);癌组织中FHIT表达降低或缺失明显高于腺瘤组(P<0.01)。结、直肠癌中p53的阳性率为51.3%,明显高于腺瘤组的10%(P<0.01)。中、晚期组(Dukes分期C+D)结、直肠癌组织p53的表达率61.7%,高于早期组(Dukes分期A+B)的34.5%(P<0.05)。结、直肠癌组织中FHIT表达降低或缺失与p53表达之间无明显相关性。结论部分结、直肠癌和少数腺瘤FHIT表达降低或缺失,提示可能在结、直肠癌的发生、发展过程中起重要作用。
Objective To investigate the expression of FHIT and p53 in colorectal carcinoma and its clinicopathological significance. Methods EnVision two-step method was used to detect the expression of FHIT and p53 in 76 cases of colorectal cancer, peri-cancerous mucosa and 30 cases of adenoma. Results The expression of FHIT was decreased or deleted in 51.3% of the patients with colorectal adenocarcinoma, 10% of the differentiated fibroadenoma in the histological grade, and 62.5% of the differentiated ones (P <0.05) The decrease or deletion of FHIT expression in adenoma group was significantly higher than that in adenoma group (P <0.01). The positive rate of p53 in junction and rectal cancer was 51.3%, which was significantly higher than that in adenoma group (P <0.01). The expression rate of p53 in rectal cancer was 61.7% in middle and advanced group (Dukes stage C + D), which was higher than 34.5% (P <0.05) in early stage (Dukes stage A + B). There was no significant correlation between reduction or deletion of FHIT expression and p53 expression in node and rectal cancer tissues. Conclusions The reduction or deletion of FHIT expression in some nodes, rectal cancer and a few adenomas suggests that it may play an important role in the occurrence and development of colorectal cancer.