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目的 :研究新城疫病毒在体外抗胃癌细胞活性及其与细胞凋亡的关系。方法 :应用倒置显微镜观察细胞形态、MTT法测NDV在体外对BGC - 82 3的抑制和杀伤作用 ,同时用流式细胞术检测胃癌细胞凋亡情况及细胞分裂周期各时象的变化。结果 :NDV在体外可使BGC - 82 3胃癌细胞形成明显的细胞病变效应、细胞生长抑制及细胞凋亡 ,且细胞凋亡率与感染时间呈正相关。G2、S期细胞减少 ,增殖指数 (PI)降低 ,与阴性对照组比较有显著性差异 (P<0 .0 1)。结论 :NDV具有显著的抗BGC - 82 3胃癌细胞活性。
Objective: To study the activity of Newcastle disease virus against gastric cancer cells in vitro and its relationship with apoptosis. Methods: The morphological changes of cells were observed by inverted microscope. The inhibition and killing effect of NDV on BGC - 82 3 cells were detected by MTT assay. The apoptosis of gastric cancer cells and the changes of cell division cycle were detected by flow cytometry. Results: NDV could induce significant cytopathic effect, cell growth inhibition and apoptosis in BGC - 82 3 gastric cancer cells in vitro, and the apoptosis rate was positively correlated with the infection time. G2, S phase cells decreased, proliferation index (PI) decreased, compared with the negative control group was significantly different (P <0.01). Conclusion: NDV has significant anti - BGC - 82 gastric cancer cell activity.