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目的：检测鼠抗人Ｇ－ｃｓｆ单克隆抗体的相对亲和力。方法：采用间接酶联免疫吸附试验（ＥＬＩＳＡ）。结果：各株单抗结合抗原的相对亲和力不同，根据５０％最大结合浓度分为２组，其中４株杂交瘤上清ＭｃＡｂｓ与纯化腹水ＭｃＡｂｓ测得结果基本吻合。结论：实验结果为合理运用这些单抗提供了重要依据。
Objective: To detect the relative affinity of murine anti-human G-CSF monoclonal antibody. Methods: Indirect enzyme-linked immunosorbent assay (ELISA) was used. Results: The relative affinities of each mAb binding antigen were different, and were divided into two groups according to the maximum binding concentration of 50%. The McAbs of the four hybridoma supernatants were basically consistent with the results of purified ascites McAbs. Conclusion: The experimental results provide an important basis for rational use of these McAbs.