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目的分析不同浓度氧乐果对运动神经元agrin基因mRNA转录水平的影响,探讨有机磷中毒突触前损害的发病机制。方法体外培养大鼠脊髓运动神经元,氧乐果染毒,检测不同浓度氧乐果对运动神经元agrin基因mRNA转录水平的影响,检测有机磷中毒是否对运动神经元的agrin基因表达产生影响。结果(1)正常组大鼠脊髓运动神经元agrin基因mRNA水平明显高于氧乐果染毒组大鼠脊髓运动神经元agrin基因mRNA水平;(2)不同浓度氧乐果染毒组(0.1mmol/L,0.01mmol/L)大鼠脊髓运动神经元agrin基因mRNA水平,各组间存在显著差异,损害程度与有机磷杀虫剂剂量存在正相关,即浓度大,agrin基因转录水平低;(3)0.1mmol/L浓度氧乐果染毒组大鼠脊髓运动神经元存活时间小于12h,0.01mmol/L浓度氧乐果染毒组大鼠脊髓运动神经元可较长时间存活。结论有机磷杀虫剂影响脊髓运动神经元agrin基因表达,且损害程度与有机磷杀虫剂剂量存在正相关,即浓度大,agrin基因转录水平低,神经肌肉接头损害严重,存在突触前损害。
Objective To analyze the effect of different concentrations of omethoate on the transcription level of agrin mRNA in motor neurons and to explore the pathogenesis of presynaptic damage caused by organophosphate poisoning. Methods Cultured rat spinal motor neurons and omethoate to detect the effects of different concentrations of omethoate on the transcriptional level of agrin mRNA in the motor neurons and examined whether organophosphate poisoning affects the expression of agrin gene in the motor neurons. Results (1) The mRNA level of agrin gene in spinal motoneurons of rats in normal group was significantly higher than that of rats in oxymeto group (2) The levels of agrin mRNA in spinal cord motoneurons of rats in different concentrations /L, 0.01mmol / L) rat agron mRNA expression level of spinal motor neurons, there are significant differences between the groups, the degree of damage and the dose of organophosphate insecticide there is a positive correlation, that is, the concentration, agrin gene transcription level is low; ( 3) 0.1 mmol / L concentration of omethoate in rats spinal motor neuron survival time is less than 12h, 0.01 mmol / L concentration of omethoate rats spinal cord motor neurons can survive longer. Conclusion Organophosphorus insecticides affect the expression of agrin gene in spinal motoneurons, and the degree of damage is positively correlated with the dose of organophosphate insecticides. That is, the concentrations of agrin gene are high, the transcription level of agrin gene is low, the damage of neuromuscular junction is serious, and the presynaptic damage .