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用反相蒸发技术制备出包裹有ＩＬ－２ＤＮＡ的脂质体，将其直接注射至小鼠腹腔中，经１０ｄ后发现，腹腔细胞体外培养的上清中含有较高水平的ＩＬ－２，腹腔巨噬细胞杀伤肿瘤细胞的活性明显增强，脾细胞体外增殖、脾细胞ＮＫ活性及体外诱导的ＬＡＫ活性均明显高于对照组；同时经脂质体转染ＩＬ－２基因的脾脏细胞具有较高的内源性ＬＡＫ活性。这表明，脂质体能将目的基因经腹腔途径直接转染体内细胞，表达出基因产物──ＩＬ－２。这种靶细胞表达的基因产物可作用于体内免疫系统，调节机体的免疫功能。本文结果为细胞因子的基因治疗的研究提供了一种简便有效的方法。
IL-2DNA-encapsulated liposomes were prepared by reverse-phase evaporation and injected directly into the abdominal cavity of mice. After 10 days, the supernatant of peritoneal cells cultured in vitro contained higher levels of IL-2, Macrophages kill tumor cells significantly increased activity of spleen cells in vitro proliferation, NK activity of spleen cells and in vitro induced LAK activity were significantly higher than the control group; at the same time liposome transfected IL-2 gene spleen cells have higher Endogenous LAK activity. This indicates that the liposome can directly transfect the target gene into cells in vivo via the intraperitoneal route and express the gene product IL-2. This target cell expressed gene products can act on the body’s immune system, regulating the body’s immune function. Our results provide a simple and effective method for gene therapy of cytokines.