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利用 1株处于CD3-CD4-CD8-阶段的病毒转化早期T细胞株C32 0研究了体外胸腺基质细胞系对早期T细胞TCR CD3复合体表达的诱导作用 ,并对参与此作用的细胞表面信号分子进行了初步鉴定 .结果显示 :在体外胸腺基质细胞系通过与C32 0细胞 细胞间相互作用 ,诱导其表面TCR_CD3分子的表达 .进一步的研究表明 ,对TCR的诱导主要作用于其转录后阶段 ;对CD3的诱导涉及转录前后两个阶段 .单抗阻断实验显示 ,抗基质细胞单抗RS2 1_C6对C32 0的上述分化表达有明显阻断作用 ,说明其识别分子参与诱导分化过程 .免疫共沉淀和蛋白印迹杂交鉴定此分子为 42ku ,是否为新型分子有待氨基酸序列分析予以确定
Using a CD3-CD4-CD8- stage virus transformed early T cell line C32 0, we investigated the induction of TCR CD3 complex in early T cells by in vitro thymus stromal cell line and investigated the cell surface signaling molecules involved in this role The results showed that the expression of TCR_CD3 on the surface of thymus stromal cell line was induced by the interaction with C32 0 cells in vitro.Further studies showed that the induction of TCR mainly in the post-transcriptional phase, The induction of CD3 involves two stages before and after transcription.MAb blocking experiments show that the anti-stromal cell monoclonal antibody RS2 1_C6 has obvious blocking effect on the above differentiation and differentiation of C32 0, indicating that its recognition molecule is involved in the induction of differentiation.Immunoprecipitation and Western blot hybridization identified this molecule as 42ku, is a novel molecule to be determined by amino acid sequence analysis