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杧果种子是顽拗型种子(RecalcitrantSeed),刚采收时含水量高达69.2～75.5%(其胚轴含水量为73.8～86.3%),在室温空气中凉干8天后,含水量下降至39.1%(胚轴含水量下降至46.5%),发芽率由100%降至0。胚轴含水量下降比整粒种子慢,这是因为大体积子叶阻碍了胚轴的脱水。杧果种子在室温空气中凉干时,随脱水程度的增加,浸种液导率从2.2μΩ·cm~(-1)·g~(-1)(单位下同)迅速上升至56.7,而胚轴中脱氢酶及酸性磷酸酶活性却迅速下降。用电扇吹风(快速干燥)42小时的杧果种子,含水量由75.5%迅速降至29.9%,浸种液电导率从1.8迅速增加至36.9,发芽率由100%降至10%。脱水损害细胞膜完整性及降低酶活性。快速脱水比慢速脱水有利,因为从电导率变化可以看出,快速脱水对膜完整性的损害不及慢速脱水严重。杧果种子的离体胚轴用硅胶干燥8小时后含水量降至11.8%,在MS+0.2mg/l BA+2.0mg/l、NAA+500mg/lGln+3%蔗糖+0.9%琼脂培养基上还有80%发芽率。将种子用电扇吹风10小时使含水量达到51.0%,将此含水量种子用塑料袋密封于15℃下贮藏7个月,还有65%发芽率。
Mangosteen seeds are Recalcitrant Seeds, with fresh water content as high as 69.2-75.5% and hypocotyls content 73.8-86.3%. After drying for 8 days at room temperature in air, the water content decreased to 39.1 % (Hypocotyl moisture content down to 46.5%), germination rate from 100% to 0. Hypocotyls decline more slowly than wholegrain seeds, because large volume cotyledon hinders hypocotyl dehydration. With the increase of the degree of dehydration, the seed soaking liquid rapidly rose to 56.7 from 2.2μΩ · cm ~ (-1) · g ~ (-1) (the same below) Axis dehydrogenase and acid phosphatase activity decreased rapidly. After drying for 42 hours with a fan, the water content decreased rapidly from 75.5% to 29.9%. The conductivity of the soaking solution increased rapidly from 1.8 to 36.9 and the germination rate decreased from 100% to 10%. Dehydration damage cell membrane integrity and reduce enzyme activity. Rapid dehydration is advantageous over slow dehydration, as it can be seen from the change in conductivity that rapid dehydration is less damaging to membrane integrity than to slow dehydration. After the dried embryos of mango seeds were dried for 8 hours, the water content decreased to 11.8% with MS + 0.2mg / l BA + 2.0mg / l, NAA + 500mg / lGln + 3% sucrose + 0.9% agar There are 80% germination rate. The seeds were blown with a fan for 10 hours to reach a water content of 51.0%. The seeds of this water content were sealed in plastic bags for 7 months at 15 ° C with a germination rate of 65%.