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目的 探讨 P53、P2 1 (WAF1 /CIP1 )基因与甲状腺癌的发生、发展及预后的关系以及它们的相互关系。方法 应用 S- P免疫组化技术 ,检测 60例甲状腺癌、1 0例正常甲状腺组织、1 0例结节性甲状腺肿、1 0例甲状腺腺瘤组织中 P53、P2 1 (WAF1 /CIP1 )的表达。结果 P53、P2 1(WAF1 /CIP1 )阳性反应仅见于甲状腺癌 ,其阳性率分别为 53.3%、41 .7% ;P53阳性率在未分化癌、有淋巴结转移或 、 期病例中增高 (P <0 .0 5) ;但 P2 1 (WAF1 /CIP1 )阳性率在未分化癌、有淋巴结转移或 、 期病例中降低 (P <0 .0 5) ;P53表达与 P2 1 (WAF1 /CIP1 )表达关系密切(P <0 .0 5)。结论 P53突变、P2 1 (WAF1 /CIP1 )缺乏均与甲状腺癌发生有关 ;P2 1 (WAF1 /CIP1 )在甲状腺癌中表达主要依赖于 P53;P53、P2 1 (WAF1 /CIP1 )在甲状腺癌中表达的变化可用于评估其生物学行为和判断预后
Objective To investigate the relationship between P53, P21 (WAF1/CIP1) gene and the occurrence, development and prognosis of thyroid cancer and their relationship. Methods S-P immunohistochemistry was used to detect P53 and P21 (WAF1/CIP1) in 60 cases of thyroid cancer, 10 cases of normal thyroid tissue, 10 cases of nodular goiter, and 10 cases of thyroid adenoma. expression. Results The positive rates of P53 and P2 1 (WAF1/CIP1) were only found in thyroid carcinoma, and the positive rates were 53.3% and 41.7% respectively. The positive rate of P53 was increased in cases with undifferentiated carcinoma, lymph node metastasis or stage (P < 0.05); but the positive rate of P2 1 (WAF1/CIP1) decreased in patients with undifferentiated carcinoma, lymph node metastasis or stage (P < 0.05); P53 expression and P2 1 (WAF1 / CIP1) expression Closely related (P < 0.05). Conclusion P53 mutation and P2 1 (WAF1/CIP1) deficiency are associated with thyroid carcinogenesis; P2 1 (WAF1/CIP1) expression in thyroid carcinoma is mainly dependent on P53; P53 and P21 (WAF1/CIP1) are expressed in thyroid carcinoma. Changes can be used to assess their biological behavior and determine prognosis