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目的:研究垂盆草醇提物对STAT-3信号通路的影响,及其诱导细胞凋亡的分子机制。方法:MTT法检测垂盆草醇提物对HepG2细胞增殖的影响;流式细胞术分析结合FITC-Annexin V/PI双标记检测对肝癌细胞凋亡的影响,免疫印迹试验检测细胞凋亡Caspase-3,Caspase-9,PARP,P-STAT-3(Tyr705),STAT-3,Bcl-2,Mcl-1相关蛋白表达水平。结果:垂盆草醇提物可明显抑制HepG2细胞的增殖,诱导HepG2细胞凋亡,且抑制作用呈剂量依赖效应。垂盆草醇提物作用后,抑制STAT-3信号转导通路,下调Mcl-1和Bcl-2的表达,引起凋亡相关蛋白Caspase-3,Caspase-9的降解/活化及PARP的降解,并呈剂量依赖效应。结论:垂盆草醇提物通过抑制STAT-3信号转导通路和Mcl-1和Bcl-2的表达,进而抑制HepG2细胞的增殖,诱导其凋亡。
OBJECTIVE: To study the effect of ethanol extract of Siniperca chuatsi on STAT-3 signaling pathway and its molecular mechanism of apoptosis. Methods: MTT assay was used to detect the effect of ethanol extract on the proliferation of HepG2 cells; flow cytometry analysis combined with FITC-Annexin V / PI double-labeled detection of apoptosis of hepatocellular carcinoma cells; Western blotting assay was used to detect the apoptosis of Caspase- 3, Caspase-9, PARP, P-STAT-3 (Tyr705), STAT-3, Bcl-2 and Mcl- Results: Alcohol extract of sarmentosum could obviously inhibit the proliferation of HepG2 cells and induce the apoptosis of HepG2 cells in a dose-dependent manner. Stilbene extract can inhibit the STAT-3 signal transduction pathway, down-regulate the expression of Mcl-1 and Bcl-2, cause the degradation / activation of apoptosis related proteins Caspase-3 and Caspase-9 and the degradation of PARP, And dose-dependent effect. Conclusion: Alcohol extract of sarmentus can inhibit the proliferation and induce the apoptosis of HepG2 cells by inhibiting the expression of STAT-3 signal transduction pathway and Mcl-1 and Bcl-2.