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目的探讨药用昆虫美洲大蠊内生放线菌的多样性和生物学特性,挖掘新的微生物资源。方法采用平板稀释法对美洲大蠊内生菌进行分离和纯化;分离菌通过形态学观察和16SrDNA序列分析进行分类鉴定,运用MEGA5.1软件,采用最大似然值法构建系统发育树,对其进行进化分析。结果从15只美洲大蠊肠道分离得到159株内生放线菌,分属于12个属9个科,其中链霉菌107株,戈登氏菌13株,诺卡氏菌2株,小单孢菌5株,栖白蚁菌4株,纤维单孢菌3株,分枝杆菌4株,微杆菌11株,短状杆菌4株,无色杆菌4株,土壤泛球菌1株,壤霉菌1株。结论美洲大蠊的内生放线菌丰富多样,其中蕴藏着的新类型放线菌,可能成为活性天然产物和先导药物的重要来源。
Objective To explore the diversity and biological characteristics of endophytic actinomycetes of the medicinal insects American cockroach and explore new microbial resources. Methods The plate-dilution method was used to isolate and purify the endophyte from American cockroach. The isolates were identified by morphological observation and 16S rDNA sequence analysis. The phylogenetic tree was constructed by MEGA5.1 software using the maximum likelihood method. Perform evolutionary analysis. Results A total of 159 strains of endophytic actinomycetes were isolated from the intestinal tract of 15 American cockroaches, belonging to 9 genera of 12 genera, including 107 Streptomyces, 13 Gordonella, 2 Nocardia, 5 strains of Alternaria, 4 strains of Termite, 3 strains of Cellulomonas, 4 strains of Mycobacterium, 11 strains of Microbacterium, 4 strains of Bacillus subtilis, 4 strains of Achromobacter, 1 strain of soil pancreas, Strain. Conclusion The endophytic actinomycetes of Periplaneta americana are abundant and diverse, and the new type of actinomycetes contained therein may become an important source of active natural products and lead drugs.