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利用Tbx18谱系示踪小鼠模型及Tbx18条件性基因敲除小鼠模型,探讨转录因子Tbx18对小鼠心血管结构发育的影响.实验建立Tbx18-Cre/Rosa26R-EYFP和Tbx18-Cre/Rosa26R-Lac Z两种基因敲入谱系示踪小鼠模型和Tbx18:Cre/Cre基因敲除小鼠模型;通过免疫荧光及X-gal染色技术,示踪Tbx18在心血管系统结构形成中的命运;通过小鼠心脏整体血管免疫组化及切片HE染色、免疫组化、免疫荧光技术,比较Tbx18:Cre/Cre基因敲除小鼠与野生型对照小鼠心脏室壁结构及冠状血管结构发育情况.示踪结果提示,Tbx18参与小鼠冠状血管及室间隔结构的形成,并与冠脉平滑肌细胞共表达;对Tbx18基因敲除小鼠及野生型小鼠的心脏结构比较提示,Tbx18基因敲除后,仍能形成形态正常的冠状血管系统,小鼠心室肌及室间隔厚度较野生型无明显差异.结果表明,Tbx18参与小鼠心脏血管平滑肌及室间隔结构的形成,但其在小鼠心脏腔室结构及冠状血管结构形成过程中不是必需的.
Tbx18 pedigree mouse model and Tbx18 conditional knockout mouse model were established to investigate the effect of Tbx18 on the development of cardiovascular structure in mice.Methods Tbx18-Cre / Rosa26R-EYFP and Tbx18-Cre / Rosa26R-Lac Z two kinds of gene knock-in pedigree model and Tbx18: Cre / Cre knockout mouse model. The fate of Tbx18 in the formation of cardiovascular system was detected by immunofluorescence and X-gal staining. Whole heart blood vessel immunohistochemistry and section HE staining, immunohistochemistry, immunofluorescence technique, Tbx18: Cre / Cre knockout mice and wild-type control mice heart ventricular wall structure and coronary vascular structure development.Tracer results Tip, Tbx18 involved in the formation of coronary vessels and septal structure in mice and co-expression of smooth muscle cells with coronary; Tbx18 gene knockout mice and wild-type mice heart structure comparison suggested that Tbx18 gene knockout, can still The formation of normal morphology of the coronary vascular system, ventricular muscle and ventricular septal thickness than the wild-type no significant difference.The results show that Tbx18 involved in the formation of cardiac vascular smooth muscle and septal structure in mice, but in the mouse heart During the cell structure is not required and coronary vascular structures form.