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目的建立通腑泻热灌肠液的质量标准及活性成分含量测定方法。方法采用薄层色谱(TLC)法对通腑泻热灌肠液中所含大黄、栀子、忍冬藤进行定性鉴别;并用高效液相色谱(HPLC)法测定通腑泻热灌肠液中大黄的2个有效成分含量。结果薄层色谱鉴别方法能定性检出大黄、栀子、忍冬藤,斑点清晰,分离度好,专属性强,且阴性对照无干扰;大黄素、大黄酚分别在0.0726~0.726μg(r=0.9998)、0.0071~0.071μg(r=0.9999)范围内与峰面积均呈现良好的线性关系,平均加样回收率分别为99.13%、103.16%,RSD(n=6)分别为2.30%、1.50%。结论所建立的定性、定量分析方法可行,专属性强,准确可靠,能有效控制通腑泻热灌肠液的质量。
Objective To establish the quality standard of Tongfu diarrhea enema and determination of the content of active ingredients. Methods TLC, TLC and HPLC method were used to qualitatively identify the rhubarb, gardenia and honeysuckle contained in Tongfu diarrhea intestinal juice, and the content of rhubarb in Tongfu diarrhea intestinal juice was determined by high performance liquid chromatography (HPLC) An active ingredient content. Results TLC method could detect rhubarb, gardenia and honeysuckle with clear spots, good resolution, strong specificity and no interference with negative control. Emodin and chrysophanol were in the range of 0.0726 ~ 0.726μg (r = 0.9998 ), 0.0071 ~ 0.071μg (r = 0.9999). The average recoveries were 99.13%, 103.16%, RSD (n = 6) were 2.30% and 1.50% respectively. Conclusion The established qualitative and quantitative analysis methods are feasible, specific, accurate and reliable, and can effectively control the quality of Tongfu diarrhea heat enema.