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目的:观察辛伐他汀对大鼠血管平滑肌细胞(VSMC)碱性成纤维细胞生长因子(bFGF)与细胞外信号调节激酶1/2(ERK1/2)蛋白表达的影响。方法:体外培养大鼠胸主动脉VSMC,MTT法测定细胞增殖活力;Western blot法检测bFGF与ERK1/2蛋白的表达。结果:与对照组比较,辛伐他汀各浓度组细胞增殖活力均显著降低(P<0.05)。与对照组比较,低、中剂量组bFGF、ERK1/2蛋白表达均显著降低(P<0.05),高剂量组bFGF、ERK1/2蛋白表达进一步降低(P<0.01);与低剂量组比较,中剂量组bFGF、ERK1/2蛋白的表达均无统计学意义(P>0.05),高剂量组则显著降低(P<0.05);高剂量组bFGF、ERK1/2蛋白的表达又较中剂量组显著降低(P<0.05),并呈剂量依赖性。结论:辛伐他汀能抑制平滑肌细胞增殖,该作用是通过抑制bFGF及其相应信号通路ERK1/2蛋白的表达来实现的,这可能是辛伐他汀治疗动脉粥样硬化、再狭窄及高血压等心血管疾病的重要机制之一。
Objective: To observe the effect of simvastatin on the expression of basic fibroblast growth factor (bFGF) and extracellular signal-regulated kinase 1/2 (ERK1 / 2) in rat vascular smooth muscle cells (VSMCs). Methods: Thoracic aorta VSMCs were cultured in vitro. The cell viability was measured by MTT assay. The expressions of bFGF and ERK1 / 2 protein were detected by Western blot. Results: Compared with the control group, the cell proliferation activity of simvastatin group was significantly decreased (P <0.05). Compared with the control group, the expressions of bFGF and ERK1 / 2 in the low and middle dose groups were significantly decreased (P <0.05), while the expression of bFGF and ERK1 / 2 in the high and low dose groups was significantly decreased (P <0.01) The expression of bFGF and ERK1 / 2 protein in the middle dose group was significantly lower than that in the middle dose group (P <0.05), but not in the high dose group (P <0.05) Significantly decreased (P <0.05), and in a dose-dependent manner. Conclusion: Simvastatin can inhibit the proliferation of smooth muscle cells by inhibiting the expression of bFGF and its corresponding signaling pathway ERK1 / 2 protein, which may be simvastatin treatment of atherosclerosis, restenosis and hypertension One of the important mechanisms of cardiovascular disease.