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目的 探讨运用MyoD基因转染使乳鼠心肌间质细胞转化为肌细胞的可能性。方法 MyoD基因是具有碱性螺旋 环 螺旋结构的生肌因子之一 ,用脂质体介导法将MyoDcDNA导入培养的乳鼠心肌间质细胞 (主要是成纤维细胞 )。结果 心脏成纤维细胞在导入MyoDcDNA 5d后可见 :①部分细胞形态发生改变 ,类似骨骼肌细胞 ,胞体伸展 ,胞浆颗粒增多 ;②经原位杂交证实 :1.8%～ 10 .9%的细胞有下游生肌分化标志即骨骼肌肌球蛋白重链mRNA的表达。结论 表明心肌成纤维细胞可转化为具有潜在功能的类骨骼肌细胞 ,提示在分子水平从心肌成形途径恢复心脏功能是有可能的
Objective To explore the possibility of using MyoD gene transfection to transform neonatal rat cardiac interstitial cells into muscle cells. Methods The MyoD gene is one of the myogenic factors with a basic helix-like helix. MyoD cDNA was introduced into cultured neonatal rat cardiomyocytes (mainly fibroblasts) by liposome-mediated method. Results Cardiac fibroblasts were observed after 5 days of introduction of MyoD cDNA: (1) some of the cells changed in morphology, similar to skeletal muscle cells, cell body extension and cytoplasmic granules increased; ② confirmed by in situ hybridization: 1.8% -10.9% of cells had downstream Myogenic differentiation markers that skeletal muscle myosin heavy chain mRNA expression. The conclusion suggests that cardiac fibroblasts can be transformed into potentially skeletal-like skeletal muscle cells, suggesting that it is possible to restore cardiac function at the molecular level from the cardiac myogenic pathway