以甲醇为溶剂,采用回流法提取荞麦蜂花粉中的黄酮类化合物。将黄酮提取物中的黄酮甙水解为黄酮甙元后,利用HPLC法测定其中槲皮素、异鼠李素含量。结果表明,采用梯度洗脱,流动相为甲醇-0.4%磷酸(pH5.12),流速1mL/min,检测波长为368nm,每克荞麦蜂花粉中槲皮素、异鼠李素的含量分别为4.9mg和0.63mg。 Using methanol as solvent, the flavonoids in buckwheat pollen were extracted by reflux method. After the flavonoid glycoside was hydrolyzed into flavone aglycone, the contents of quercetin and isorhamnetin were determined by HPLC. The results showed that the mobile phase consisted of methanol-0.4% phosphoric acid (pH 5.12), the flow rate was 1 mL / min and the detection wavelength was 368 nm. The contents of quercetin and isorhamnetin per gram of buckwheat pollen were 4.9 mg and 0.63 mg.